Microfluidics-based Double Emulsion Synthesis Services

Creative Biolabs' microfluidic technology enables the individual generation of double-emulsion droplets through precise manipulation of fluids within micron-scale channels. This ensures exact control over the core/shell structure, size, and composition of each droplet, delivering monodispersity and reproducibility unmatched by conventional methods.

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Fig. 1 Simplified microfluidic system. (Wei, et al., 2005)

At Creative Biolabs, we provide cutting-edge microfluidics-based double emulsion synthesis services to support advanced research in drug delivery, biomaterials, diagnostics, and encapsulation technologies.

Our platform ensures high reproducibility, narrow size distribution, and scalable production, making it ideal for encapsulating proteins, peptides, nucleic acids, nanoparticles, and other sensitive payloads. In addition to standard preparation, we offer tailored formulation design, stability testing, leakage evaluation, and release kinetics profiling.

Our Service Portfolio

Our services are built on a "customer-centric" framework, ensuring alignment with your goals, regulatory needs, and budget.

Custom Formulation Engineering

We have experts in formulation research to create custom-made formulations for you. By optimizing the components of each phase, selecting appropriate emulsifiers, and calibrating key parameter indicators, we balance the functionality, stability, and biocompatibility of the emulsion.

Scalable Manufacturing

We bridge lab-to-industry gaps with three processes:

High-Shear Emulsification	Suitable for emulsion systems requiring moderate uniformity, applicable for medium-to-large volume batches.
Microfluidic Emulsification	Applicable for small-volume batches with low coefficient of variation; suitable for targeted drugs, and mostly used with microchannels made of PDMS or glass.
Membrane Emulsification	Suitable for sensitive ingredients, its gentler processing method can preserve the activity of most enzymes.

Post-Processing & Quality Assurance

We solidify emulsions through processes such as cooling, cross-linking, or spray drying. Subsequent quality testing involves a range of professional methods, including SEM for morphology analysis, laser diffraction for particle size measurement, HPLC for encapsulation efficiency determination, and USP dissolution testing for release profile assessment.

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Service Advantages

Unmatched Expertise

Our expert scientists have collaborated with renowned universities on emulsion synthesis R&D initiatives. Additionally, we have partnered with top pharmaceutical companies, providing them with temperature-responsive emulsions to support the development of new effective treatment methods for diseases.

Cost Efficiency

Choosing us to assist with your emulsion synthesis can save you most of the equipment costs. Additionally, we offer flexible pricing models and are confident in providing a pricing range that meets your expectations, helping you cut unnecessary expenses.

Quality & Consistency

We have a dedicated quality standard evaluation system, and all production processes strictly adhere to this set of regulations. The batch-to-batch variation of our products is far lower than the industry average.

Service Process

Process	Description
Needs Assessment	When you reach out to us for consultation, our expert team will conduct a feasibility study as soon as possible based on your requirements.
Microfluidic Platform Setup	Based on the project design, we select or customize microfluidic chip structures (e.g., co-flow, flow-focusing, T-junction) and optimize flow parameters to ensure the formation of monodisperse droplets with desired size and stability.
Formulation Preparation	We prepare the aqueous and oil phases with the appropriate surfactants, solvents, and stabilizers. Payloads such as proteins, peptides, nanoparticles, or dyes are incorporated into the inner phase for controlled encapsulation.
Double Emulsion Generation	Using precision microfluidic devices, we synthesize highly uniform double emulsions, adjusting droplet size, shell thickness, and internal compartment composition with fine-tuned control.
Quality Control & Characterization	Each batch undergoes rigorous testing, including size distribution analysis, encapsulation efficiency, stability profiling, and leakage evaluation.
Reporting & Delivery	Clients receive a comprehensive report covering experimental conditions, data analysis, raw images, and interpretation of results, along with formulated samples when required.

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Key Applications

Microfluidics-based double emulsions provide a versatile and precisely controllable platform for encapsulation and delivery across multiple research and industrial domains. At Creative Biolabs, our services empower clients to explore innovative solutions in:

Drug & Gene Delivery: Highly uniform double emulsions serve as carriers for small molecules, peptides, proteins, nucleic acids, and nanoparticles, offering controlled release profiles and enhanced stability.
Vaccine & Immunotherapy Formulation: Double emulsions act as effective templates for antigen encapsulation and adjuvant delivery, supporting next-generation vaccine design and immune modulation studies.
Biomaterials & Tissue Engineering: Our systems enable encapsulation of bioactive factors and living cells within tunable hydrogel shells, advancing the design of scaffolds and cell-laden constructs for regenerative medicine.
Diagnostics & Biosensors: Double emulsions can encapsulate enzymes, fluorescent probes, or nanoparticles to create miniaturized reaction compartments for sensitive, multiplexed diagnostic platforms.
Food & Nutraceutical Research: Encapsulation of flavors, probiotics, vitamins, and bioactive compounds in double emulsions improves stability, solubility, and controlled release in food and nutraceutical applications.
Cosmetic & Personal Care Formulations: Double emulsions allow for sustained delivery of active ingredients such as antioxidants, peptides, and moisturizing agents, enhancing product performance and shelf life.

Client Testimonials

“We needed a reliable way to encapsulate fragile enzymes for a biosensor project. Most conventional methods led to rapid enzyme inactivation. The Creative Biolabs team tailored the oil phase and surfactant system to protect enzyme activity, and the resulting double emulsions remained functional over weeks of storage."

— Dr. Elena Marquez, Senior R&D Scientist

"In our preclinical vaccine study, we needed antigen-loaded double emulsions with high encapsulation efficiency and reproducibility. Creative Biolabs delivered emulsions with >90% encapsulation and minimal leakage."

— Jake Bennett, Product Development Manager

"We approached Creative Biolabs to help encapsulate probiotics in double emulsions to enhance survival during GI transit. The emulsions they produced not only had excellent uniformity but also significantly improved probiotic viability under simulated gastric conditions."

— Sophia Chen, Formulation Lead

"The Creative Biolabs microfluidic process allowed us to create stable double emulsions with protective shells that preserved activity for months. Their data package included size distribution curves, stability studies, and even guidance on storage conditions, which proved invaluable for product development."

— Mark Wilson, European Cosmetics Company

"When designing a drug delivery study, we required long-term release profiling. Creative Biolabs generated customized W/O/W double emulsions with tunable shell thickness, and their release studies under different pH and temperature conditions gave us comprehensive data for comparison."

— Lisa Torres, Director of Translational Medicine

Published Data

Facile synthesis of surface-modified hollow-silica (SiO₂) aerogel particles via oil-water-oil double emulsion method

In one study, the oil-water-oil (OWO) double emulsion method was implemented to synthesize surface-modified hollow silica (SiO₂) aerogel particles in a facile and effective way. An emulsion-based synthesis technique is a simple and generally secure approach to producing a wide range of hollow particles with precisely controlled particle size, shape, composition, and spatial arrangement. Water droplets stabilized by surfactants in oil solution act as templates for the hollow silica particles. A silica shell was produced by hydrolysis and polymerization between a silica precursor such as tetraethyl orthosilicate (TEOS) dissolved in the oil phase with water droplets on a W/O interface.

Fig. 1 A simple route for the synthesis of hollow silica aerogel particles using OWO liquid strategy and solvent exchange process.^1,3

Synthesis of polymeric nanoparticles by double emulsion: encapsulation of antibiotics

Polymer nanoparticles (PNPs) synthesized via Double emulsion (DE) method, also known as water-in-oil-water emulsion (W/O/W), consists of complex nanostructures designed to encapsulate active pharmaceutical ingredients. This technique involves dispersing aqueous droplets within oil or oil/polymer spheres, which are then embedded in an aqueous phase. The key feature of this structure lies in its ability to encapsulate diverse molecules within a single nano-system. Antibacterial drugs coated using this dual emulsion synthesis method can enhance the controlled release of active pharmaceutical ingredients (APIs) at infection sites, thereby increasing drug concentration while minimizing adverse effects.

Synthesis of polymeric nanoparticles by double emulsion. (OA Literature) Fig. 2 Preparation of polymeric nanoparticles via double emulsion technique.^2,3

References

Kapadnis, Pratik S., et al. "Facile Synthesis of Surface-Modified Hollow-Silica (SiO2) Aerogel Particles via Oil–Water–Oil Double Emulsion Method." Gels 10.6 (2024): 380. https://doi.org/10.3390/gels10060380
de Almeida Campos, Luís André, et al. "Synthesis of polymeric nanoparticles by double emulsion and pH-driven: encapsulation of antibiotics and natural products for combating Escherichia coli infections." Applied Microbiology and Biotechnology 108.1 (2024): 351. https://doi.org/10.1007/s00253-024-13114-5
Distributed under Open Access license CC BY 4.0, without modification.

Created September 2025

FAQs

Q: What types of double emulsions can you generate using microfluidics?

A: We specialize in producing highly uniform W/O/W (water-in-oil-in-water) and O/W/O (oil-in-water-in-oil) emulsions. Both types can be tailored for different payloads, such as proteins, peptides, nucleic acids, nanoparticles, probiotics, or active cosmetic ingredients.

Q: Can you help customize the emulsion size and shell thickness?

A: Yes. We can fine-tune flow rates, chip geometry, and surfactant systems to control droplet diameter (from a few microns to hundreds of microns) and shell thickness, meeting your specific formulation needs.

Q: What information do you need from clients before starting a project?

A: We usually request payload type, desired emulsion configuration, preferred solvents/surfactants, stability requirements, and intended application. With this, we can design a customized project plan.

Q: Are your services suitable for both research and industrial projects?

A: Yes. We support early-stage academic research, proof-of-concept studies, and pilot-scale projects. Our team can also provide process transfer guidance for clients moving toward industrial production.

Q: Can your double emulsions be designed to release ingredients only under specific environmental conditions, like certain pH levels?

A: Yes. We can engineer the emulsion's middle phase to respond to stimuli like pH, enabling targeted release of active ingredients under your desired conditions.

Q: What is the typical turnaround time for a project?

A: Turnaround varies with complexity, but most projects are completed within 2–6 weeks, including consultation, synthesis, testing, and reporting. Expedited services may be available.

Q: How do I initiate a project with Creative Biolabs?

A: Simply contact us through our online inquiry form or email. Our specialists will schedule a consultation, draft a project proposal, and guide you through sample submission and study initiation.

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Our double emulsion synthesis services provide the expertise, speed, and scalability to turn your vision into reality. Book a free 30-minute consultation now—our scientists will assess your project, share tailored solutions, and help you stay ahead of competitors.