Based on our advanced microfluidic platforms and powerful technology, Creative Biolabs provides custom microfluidic chip development services to meet our customers’ different purposes, greatly improving the accuracy and sensitivity of the detection. Our highly skilled scientists are ready to get started on your projects.
Immunoassay is one of the most widely used biomedical diagnostic methods. It is based on the specific binding of an antibody to an antigen. Immunoassays are used to quantify proteins and small molecules in different fields such as medical diagnostics, proteomics, pharmaceutical research, and biological research. Automation of immunoassay tests generally requires a complex and cumbersome robotic technique for fluid handling, which is very expensive and not a practical tool for point-of-care testing. Thus, the rapid development of microfluidic-based immunoassay technology lights a pathway for diagnostics in recent years.
Microfluidic chips have been developed to perform various immunoassays that can be employed to detect biological agents. Microfluidic immunoassays make use of a network of microchannels and/or immunoreactive chambers and allow integration of multiple liquid handling processes required in diagnostic assays, such as pumping, metering, sampling, dispensing, and sequential loading and washing. Overall, the microfluidic immunoassay platform is an emergent, powerful option for analyzing a wide variety of analytes in different fields (e.g., medical diagnosis, pharmaceutical, and biological research, food industry, and environmental applications).
In general, microfluidic immunoassay has two main formats:
In heterogeneous immunoassays, antibodies are either immobilized on the surface of the microfluidic chip or micron-dimension beads embedded in the device. The heterogeneous microfluidic immunoassay generally confers high sensitivity and excellent specificity.
In homogeneous immunoassays, bound and unbound antibodies are mostly discriminated against by their electrophoretic ratio mobility in microchannels.
Fig.1 PDMS-based microfluidics immunoassay chips. (Mou, 2017)
Microfluidic immunoassay can not only detect single-antigen but also can serve as a multivariate immunoassay based on different strategies, such as:
Surface microarray is one of the most common platforms used for multiplexed immunoassays. This platform relies on its reduced size to increase the number of analytes analyzed from a single sample, and microfluidic techniques could facilitate sample handling for these platforms.
Microbeads are frequently used in microfluidic immunoassays and serve as a simple mechanism to reproducibly deliver antibodies to desirable locations. Microbeads can be non-magnetic or magnetic, and different antibody-immobilization methods can be implemented.
Using microfluidic systems for immunoassay has become a popular solution. For over ten years, Creative Biolabs has been a leading supplier of custom microfluidic solutions to our partners worldwide. To meet the growing demand for microfluidic technology in immunoassay, we develop a suite of microfluidic services for immunoassay with high specificity, sensitivity, and reproducibility. Creative Biolabs is pleased to share our extensive expertise in microfluidic technology in facilitating our clients’ research and project development. If you do not find a specific service item you are looking for, please directly contact us. We can design and customize your project and generate the exact microfluidic chips you need.
For Research Use Only. Not For Clinical Use.