Organ-on-chip Cell Culture Platform

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Overview Features Application Platform Components Specification

Introduction

Creative Biolabs provides you with a full range of microfluidic organ-on-a-chip and cell culture instruments and services to facilitate the start of your research to the greatest extent. If you are overwhelmed by starting a microfluidic cell experiment from scratch, Creative Biolabs' customized platform for cell culture can perfectly solve your problem.

Fig.1 Introduction of microfluidic cell culture platform. (Creative Biolabs Original)

Microfluidic organ-on-chip/cell culture platform is a breakthrough tool in modern cell biology and biomedical research, providing scientists with a precisely controlled culture environment that allows them to simulate the growth conditions of tissues and cells in vivo, which can be used for a variety of applications, including organ chip research, drug screening and toxicity testing. Through microfluidics, we can supply culture fluid in real time, regulate temperature and atmosphere, and create complex three-dimensional cell structures, providing biologists, drug developers and medical researchers with a powerful tool to better understand cell behavior, disease mechanisms and new drug development.

Fig.2 Components of microfluidic cell culture platform. (Creative Biolabs Original)

Features

High-precision pressure-driven pump module, dedicated to generating uniform, pulse-free trace liquid or gas, can ensure the accuracy and stability of liquid/gas injection process, with fast response time and high stability, and can realize complex microfluidic manipulation such as multi-reagent sequence injection and circulation.

The flow sensor can be used alone or in conjunction with the pressure-driven pump module. It can be placed in any microfluidic experimental system to monitor the flow at a specified point in real time. The patented algorithm ensures the stability, accuracy and reliability of flow monitoring.

It can realize heating, cooling and constant temperature. Combined with PID control algorithm and high-resolution power output, the temperature control is stable.

Supports gas concentration control such as CO2, N2, etc. The premixed gas flow rate control range is: 0.6-1 L/min, and the adjustment accuracy is 1%

The maximum working flow is 5L/min, and the humidity control range is 5%~95%.

Application

With the appropriate microfluidic chip, the platform can realize common 2D/3D culture, which is suitable for epithelial cell culture, toxicity testing, absorption testing, air-liquid interface (ALI) culture and other studies.

Recommended chips: BE-Flow Standard (CAT#: MFMM1-GJS1), BE-Transflow Standard (CAT#: MFMM1-GJS3)

Fig.3 Microfluidic chips for cell culture. (Creative Biolabs Original)

The platform can be used for concentration gradient culture in 3D cell culture. This chip consists of a central cell culture chamber and three long channels connected to the central chamber. 2D culture can also be performed in the channels.

Recommended chips: BE-Gradient Standard (CAT#: MFMM1-GJS2)

Fig.4 Microfluidic chips for concentration gradient culture. (Creative Biolabs Original)

With the Be-Doubleflow chip, the two channels inside are connected by a permeable membrane with optional pore size to facilitate the control of the interaction rate during the experiment. It is suitable for application scenarios that require circulation, such as research on metabolite accumulation, drug concentration attenuation, bacterial infection, circulating tumor cells, etc. It is also suitable for 2D or 3D culture in a simulated bionic environment.

Recommended chips: BE-Doubleflow Standard (CAT#: MFMM1-GJS4)

Fig.5 Microfluidic chips for cell culture circulation culture. (Creative Biolabs Original)

Platform Components

Fig.6 Environmental chamber module. (Creative Biolabs Original)

Environmental chamber module

  • ITO glass is used to ensure uniform temperature during heating
  • The chamber is highly sealed to maintain stable temperature, humidity and atmosphere
  • Contains microfluidic chip adapter fixture to adapt to chips of specified size
  • Aluminum alloy anti-corrosion chamber
  • Highly transparent quartz observation chamber
  • Quick-plug electrical interface and air inlet, easy to connect, safe and reliable

Fig.7 Fluid drive module (Creative Biolabs Original)

Fluid drive module

  • Stable output and high repeatability
  • The pressure output is pulse-free, avoiding the disadvantage of slow response of the injection pump
  • Equipped with a flow meter that can monitor flow in real-time, achieving constant flow control
  • The flow rate can reach nl/min level under certain conditions,
  • The pump body does not contact with the reagent, avoiding cross contamination.

Fig.8 Liquid storage workstation. (Creative Biolabs Original)

Liquid storage workstation

  • Automatic selection and switching of up to 10 reagents
  • Circulation of quantitative liquids
  • Small internal volume of the switching valve and no dead volume
  • Equipped with automation software to achieve fully automated control of fluid manipulation

Fig.9 Temperature monitoring module. (Creative Biolabs Original)

Temperature monitoring module

  • Stable temperature control
  • Temperature control supports room temperature 50℃, which can meet most culture needs
  • 3-channel design, can provide an additional temperature measurement
  • With semiconductor refrigeration module, support culture medium long-term storage at 4-8℃
  • Equipped with software, easy to operate

Fig.10 Atmosphere humidity control module. (Creative Biolabs Original)

Atmosphere humidity control module

  • Adopts digital mass flow meter with high control accuracy
  • Can control any proportion of O2, CO2 and N2
  • With CO2 and O2 concentration sensors, can display concentration values in real time
  • With humidity sensor, supports real-time display of humidity in environmental chamber

Specification

The parameters listed below are general standard product parameters. For specific selection and customization, please contact us.

Number of reagents for sequential injection Up to 10
Reagent control function Cyclic injection, sequential injection, quantitative injection
Temperature control accuracy ±0.1℃
Temperature control range Room temperature~50℃
Premixed gas flow rate control range 0.6-1 L/min
CO2 concentration control ratio 0-15%
Gas mixing manual adjustment accuracy 1%
Humidification module working flow Maximum 5 L/min
Humidity control performance Range 5%-95%
Pressure range 0-1000mbar
Number of pressure outputs 8 outputs (including 4 independent outputs)
Pressure stability <0.1% CV (measured value)
Response time As low as 40 ms
Settling time As low as 100 ms
Gas source Non-corrosive or non-toxic gas (compressed air, N2, CO2)
Operating temperature 5-40℃
Model Sensor inner diameter Maximum pressure resistance Flow range Flow control accuracy
XS 25 μm 200 bar 0±1.5 μL/min >75 nL/min, 10% m.v.
<75 nL/min, 7.5 nL/min
S 150 μm 200 bar 0±7 μL/min >0.42 μL/min, 5% m.v.
<0.42 μL/min, 21 nL/min
M 430 μm 100 bar 0±80 μL/min >2.4 μL/min, 5% m.v.
<2.4 μL/min, 0.12 μL/min
L 1 mm 12 bar 0±1 mL/min >0.04 mL/min, 5% m.v.
<0.04 mL/min, 1.5 μLl/min
XL 1.8 mm 5 bar 0±5 mL/min >0.2 mL/min, 5% m.v.
<0.2 mL/min, 10 μL/min
M+ 400 μm 12 bar 0±2 mL/min >10 μL/min, 5% m.v.
<10 μL/min, 0.5 μL/min
L+ 400 μm 12 bar 0±40 mL/min >1 mL/min, 5% m.v.
<1 mL/min, 5% m.v.

Creative Biolabs provides comprehensive microfluidic organ-on-a-chip/cell culture solutions. We can provide customized services to build a system that suits your experimental requirements. Please contact us for more details.

For Research Use Only. Not For Clinical Use.

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