Impedance Analysis-Based Sensor Development Service
Inquiry
Cutting-edge electrochemical knowledge allows Creative Biolabs to develop and fabricate microfluidic impedance biosensing devices for our clients, which are effective and promising methods for biosensor-based detection of biomolecules/cells.
Microfluidic Impedance Sensor
The impedance detection principle is based on the classical immunological coupling reaction. Biological antibody molecules are pre-bonded to the surface of the conductive/semiconductor transducer in the microfluidic channel, and the solution to be tested containing the antigen is then injected into the channel for detection. Biomolecules and analytes form specific complexes on the surface of the transducer, thereby changing the impedance characteristics of the conductive transducer. The measured impedance is composed of medium impedance and interface impedance, and the establishment of the equivalent circuit will help to determine the mechanism of system impedance change. Its sensitivity is directly related to the capture ligand's availability, particle enrichment level, and transducer impedance signal conversion efficiency.
Fig.1 3D schematic of a microfluidic impedance sensor. (Dastider, et al., 2015)
The impedance biosensor can convert the concentration of the analyte into an electrochemical impedance signal and output a quantitative impedance spectrum. The intelligent microfluidic chip integrates the enrichment, capture, analysis and other functions of the analyte in a tiny volume, which promotes the miniaturization and automation of impedance sensors. Microelectrodes with lower ohmic resistance enable fast impedance measurements with high sensitivity and high signal-to-noise ratio.
Fig.2 Impedance-frequency and phase angle-frequency curves under different solution concentrations. (Li, et al., 2022)
Although microfluidic impedance sensors are still in the early stages of exploration, our optimized technologies, advanced processing techniques and operating protocols have brought stronger and more comprehensive technical support to impedance sensors.
Dielectrophoretic impedance technology utilizes the electrokinetic movement of dielectric materials in a non-uniform electric field to achieve a highly controllable detection process. Precise control of the solution to be tested allows for low detection limits, parallel monitoring and high-throughput detection.
Nanomaterials have higher specific surface area with excellent electrocatalytic activity and electronic properties. Nanoparticles and nanotubes have been widely used as immobilization substrates for bioreceptors to improve the response characteristics of impedance sensors and amplify the detection signal.
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Microfluidic Impedance Cytometry
Impedance cytometry chips use microelectrodes to measure the impedance of individual particles. Particles with specific dielectric properties pass through the detection area at high speed in suspension and generate electrical signals that are directly related to the particle impedance. This label-free cell separation/discrimination technique distinguishes bioparticles with different electrical properties at extremely high flow rates.
Our Services
Thanks to our cutting-edge electrochemical microfabrication technology and biological knowledge, Creative Biolabs provides development and construction services for microfluidic impedance biosensors. With our help, you may skip the highly complex manufacturing techniques and tedious prototype testing, and move directly from the design of the electrical structural unit to important downstream experiments. You may take advantage of the multiple benefits and technical support of microfluidic impedance sensors to indulge in exploring the infinite possibilities of impedance devices in the field of point-of-care diagnosis and in situ detection. Please don't hesitate to contact us with any demands.
References
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Dastider, S.G.; et al. Efficient and rapid detection of salmonella using microfluidic impedance based sensing. Journal of Sensor. 2015, 8: 293461.
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Li, C.; et al. Detection of tomato ringspot virus based on microfluidic impedance sensor. Micromachines. 2022, 13: 1764.
For Research Use Only. Not For Clinical Use.
