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Microfluidic systems provide an ideal platform and unprecedented prospects for developing in situ diagnostic solutions. Creative Biolabs provides our clients with attractive point-of-care (PoC) in situ sensor development services with rich functional diversity and reasonable cost.

Why Choose Microfluidic PoC System?

The point of biological research is the transfer and expansion from the laboratory environment to the clinical field. Although many medical testing experiments with clinical significance can be carried out perfectly in the laboratory environment with perfect outcomes, they cannot be performed by on-site tools in practical applications. This setback greatly limits the development of medical testing. WHO has proposed affordable, sensitive, specific, user-friendly, robust and fast, equipment-free and deliverable to end-users ("ASSURED") criteria to measure the pros and cons of PoC devices.

The reliability and miniaturization of microfluidic systems allow precise measurement of small sample volumes. Our optimized microfabrication technology allows the combination of microfluidic systems and PoC components, which provides unparalleled advantages for the development of in situ sensors.

• Portability, can be used for analysis and detection outside the laboratory;
• Improved detection speed by the microfluidic system;
• Miniaturized equipment greatly reduces sample consumption;
• Fully automated, eliminating human interference and contamination;
• Efficient repeated testing;
• Parallel experiments and multiplexing;
• Provides early diagnosis for patients;
• Adaption to multiple detection environments.

Cutting-edge microfabrication technology platforms and optimized microfluidic system processing protocol allow Creative Biolabs to provide our clients with compact and low-cost solutions for PoC devices by means of microfluidic integration. With our help, you may explore the vast future of microfluidic PoC in situ sensors and, in the process, leverage their strengths. Based on different detection principles, there are several development directions for microfluidic PoC sensors.

Lateral Flow Assay (LFA)

LFA devices contain a sample pad to absorb the biological sample, a nitrocellulose detection zone that allows antigen-antibody interaction/DNA hybridization and an absorbent to collect excess reagents. The most common LFA PoC sensor is a paper-based colorimetric immunoassay device, which has been commercialized and used for rapid HIV testing, pregnancy testing, and various virus detection. It is worth noting that paper-based microfluidic PoC devices have also been well-applied during the Covid-19 pandemic. The paper-based in situ detection device uses body fluid as a small-volume sample, and obtains fast and repeatable results without any additional equipment and expertise, fully meeting the ASSURED standards specified by WHO.

Fig.1 Schematic of LFA device. (Sachdeva, et al., 2021)

Nucleic Acid Amplification

The polymerase chain reaction is the gold standard for high-sensitivity molecular quantitative detection. Microfluidic-based in situ nucleic acid detection provides unparalleled specificity for disease monitoring and molecular diagnosis while maintaining the miniaturization and sensitivity of PoC, an isothermal amplification technique allows nucleic acid amplification in situ sensors driven by simple and reusable heating units.

Fig. 2 Microfluidic PoC device. (Mauk, et al., 2018)

Electrochemical Assay

Dielectrophoresis (DEP) is a label-free, fast and sensitive means of ion manipulation. The DEP unit allows microfluidic in situ sensors to perform operational analytical detection using minimal amounts of biological samples. Classic three-electrode/two-electrode configuration allows miniaturization and conversion of virtually any immunocoupling assay into a high-quality electrochemical report system.

Our Services

Creative Biolabs provides a one-stop solution for microfluidic PoC in situ sensor development. You may take full advantage of our advanced microfabrication platform and obtain more economical, easier and faster manufacturing technology. We can develop and construct PoC in situ sensors according to your needs and designs. If you expect to devote your precious time to other important work, we are also willing to carry out prototype testing or other downstream detection projects. With our help, you may effectively advance research on disease diagnosis and bioassay at a reasonable cost, so don't hesitate to contact us for more information.

References

1. Sachdeva, Davis, et al. " Microfluidic point-of-care testing: commercial landscape and future directions. " Frontiers in Bioengineering and Biotechnology 8 (2021): 602.
2. Mauk, Song, et al. " Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests " Biosensors 8.1 (2018): 17.

For Research Use Only. Not For Clinical Use.