Uterus-Organ-On-A-Chip Model Development Service
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Background Uterus-on-a-chip Applications Why Choose Us? FAQs Products
Creative Biolabs' Uterus-On-A-Chip Model Development Service offers a comprehensive solution for researchers seeking to investigate uterine function, disease mechanisms, and therapeutic interventions. We provide customized microfluidic devices that accurately replicate the key structural and functional aspects of the human uterus, enabling you to conduct more physiologically relevant studies.
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Background
Situated in the pelvic region between the bladder and rectum, the uterus comprises two anatomical regions: the corpus and cervix. Superiorly, it interfaces with the fallopian tubes, while its inferior cervical canal connects to the vaginal passage. This hollow, muscular structure features a tri-layered wall—an outer serosal membrane, a middle myometrial layer of smooth muscle, and an inner endometrium that cyclically remodels under ovarian hormonal regulation. The endometrium harbors diverse cellular populations, including lumen-facing and glandular epithelia, stromal fibroblasts, immune mediators, and specialized vasculature forming spiral arterioles, all coordinating its periodic regeneration and shedding during menstrual phases.
Fig 1. Diagram of the human uterus and surrounding structures. Distributed under CC BY-SA 4.0, from Wiki, without modification.
Uterus-on-a-chip
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Uterus-on-a-chip for studying fertilization and embryonic development
A breakthrough uterine microfluidic system now enables the replication of ovulation-to-embryogenesis processes for enhanced IVF applications. This bioreactor employs PDMS bilayers sandwiching a porous polycarbonate membrane that hosts endometrial cultures. The upper zigzag microchannel utilizes staggered microtraps for oocyte sequestration, while the lower perfusion network features micropillar-stabilized fluidic channels. By facilitating gamete-stromal cross-talk and transmembrane molecular exchange, the platform demonstrated 38% higher blastocyst yields than static cultures. Performance enhancements derive from: 1) Perfusion-maintained metabolic homeostasis via nutrient cycling/waste removal; 2) Membrane-enabled embryotrophic signaling through soluble endometrial factors. This organomimetic approach revolutionizes fertility treatments by reconstituting endometrial-embryonic dialog critical for successful implantation.
Fig 2. Mimicking the uterus and endometrium on the chip.1,3
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Microengineered Vascularized Endometrium Chip for Studying Endometrial Vasculature
During the luteal phase of gestation, progesterone drives endometrial stromal remodeling—a physiological adaptation where fibroblasts acquire secretory and immunomodulatory capacities to support conceptus implantation and placentation. This stromal transformation represents a critical juncture in early pregnancy establishment. Recent advances employ microphysiological systems to replicate this dynamic milieu. Gnecco et al. engineered a triphasic endometrial model co-culturing endometrial stromal cells (ESCs) with vascular endothelia, synchronizing with 28-day hormonal rhythms.2,3 Stromal commitment was quantified through prolactin secretion kinetics and cytoskeletal reorganization. The platform concurrently mapped vascular network maturation and paracrine crosstalk dynamics during decidualization. This hormone-responsive construct maintained functional stability for 28 days, enabling longitudinal analysis of biochemically induced endometrial-vascular interplay crucial for pregnancy maintenance.
Fig 3. Characterization of co-culture of endometrial stromal fibroblasts (stroma) and HUVECs in the two-chamber device.2,3
Applications
Uterus-on-a-chip models have a wide range of applications in reproductive biology research and drug development, including:
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Modeling Endometrial Receptivity: Studying the complex interactions between the endometrium and the developing embryo to identify factors that influence successful implantation.
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Investigating Uterine Diseases: Recreating pathological conditions such as endometriosis, adenomyosis, and uterine fibroids to understand their pathogenesis and identify potential therapeutic targets.
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Evaluating Drug Candidates: Testing the efficacy and safety of new drugs for treating uterine disorders in a more physiologically relevant environment.
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Studying Hormone Action: Examining the effects of hormones on uterine cell function and identifying mechanisms of hormone resistance.
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Developing Personalized Medicine Strategies: Tailoring treatments for uterine disorders based on individual patient characteristics and responses.
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Understanding Placental Function: Modeling the interactions between the uterus and placenta to study pregnancy complications and improve maternal-fetal health. As noted in recent research, OOC technology is being used to model "placental drug transport".
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Why Choose Us?
Creative Biolabs is a leading provider of advanced organ-on-a-chip solutions, with a proven track record of delivering high-quality, customized models to researchers across various fields. Our Uterus-On-A-Chip Model Development Service offers several key advantages:
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Physiological Relevance: Our models accurately mimic the complex microenvironment of the human uterus, providing a more physiologically relevant platform for your research compared to traditional 2D and 3D cell cultures.
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Customization: We work closely with you to design and fabricate Uterus-On-A-Chip models that meet your specific research needs, including the selection of cell types, model complexity, and experimental parameters.
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Expertise: Our team comprises experienced scientists and engineers with extensive expertise in microfluidics, cell biology, and reproductive biology.
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Advanced Technology: We utilize state-of-the-art microfabrication techniques and advanced imaging and analytical tools to ensure the highest quality and reproducibility of our Uterus-On-A-Chip models.
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Comprehensive Support: We provide comprehensive support throughout the entire process, from initial consultation to data analysis and reporting.
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Published Data: Our Uterus-On-A-Chip models have been validated in numerous studies, demonstrating their accuracy, reliability, and predictive power.
FAQs
Q1: How do Creative Biolabs' uterine organ-on-chip systems advance beyond conventional cell culture platforms?
A1: Our engineered uterine models surpass static 2D/3D cultures through biomechanical replication of the endometrial niche—integrating 3D multicellular architecture, endocrine signaling gradients, and hemodynamic perfusion. This systems biology approach yields organ-level functional readouts unobtainable via traditional monolayer or spheroid methods.
Q2: Is customization available for these uterine models?
A2: Our core expertise lies in the adaptive engineering of reproductive organ-on-chip platforms. Through collaborative design processes, we optimize cellular compositions (primary/patient-derived lineages), hormone cycling protocols, and perfusion parameters to align with your investigational targets—from endometriosis modeling to contraceptive pharmacodynamics.
Q3: How does this uterine-on-a-chip system advance pharmacological research?
A3: The system transforms drug development through biomimetic emulation of uterine functions, supporting high-throughput pharmacodynamic profiling with human physiological precision. By mirroring endometrial-vascular interactions, it reduces preclinical animal reliance by >60% while accelerating lead compound validation.
Recommended Products
For researchers facing challenges in initiating microfluidic cellular investigations de novo, Creative Biolabs' engineered cell-culture systems deliver integrated solutions that streamline workflow bottlenecks.
CBLcell™ Organ-on-chip Cell Culture Platform
Creative Biolabs provides you with a full range of microfluidic organ-on-a-chip and cell culture instruments and services to facilitate the start of your research to the greatest extent. If you are overwhelmed by starting a microfluidic cell experiment from scratch, Creative Biolabs' customized platform for cell culture can perfectly solve your problem.
Our chips offer the freedom to choose the cell seeding channels and perfusion conditions, enabling various cell culture modes.
For more information about Creative Biolabs products and services, please contact us.
References
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Deng, Zhi-Min, et al. "Organ-on-a-chip: future of female reproductive pathophysiological models." Journal of Nanobiotechnology 22.1 (2024): 455.
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Gnecco, Juan S., et al. "Compartmentalized culture of perivascular stroma and endothelial cells in a microfluidic model of the human endometrium." Annals of Biomedical Engineering 45 (2017): 1758-1769.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not For Clinical Use.
