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Creative Biolabs' Oviduct-On-A-Chip Model Development Service provides you with tailored microfluidic systems designed to replicate the intricate physiological conditions of the oviduct. This enables more accurate and predictive studies of reproductive processes, disease mechanisms, and the effects of therapeutic interventions. We deliver custom-engineered solutions to meet your specific research needs.

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Background

The oviduct serves as a critical biosystem orchestrating early gestational events through specialized microenvironments facilitating gamete fusion, embryogenesis, and embryonal transit to the uterus. Its segmented architecture exhibits distinct biophysical and secretory characteristics essential for preimplantation development. At the ampullary-isthmic interface, coordinated mucociliary activity, contractile dynamics, and mucosal secretions mediate zygote formation and directional transport. Tubal epithelia further secrete implantation-critical paracrine factors like growth mediators and immunomodulators. Current experimental limitations persist in replicating this organ's structural complexity (multizonal histology) and biochemical sophistication (dynamic fluidic/endocrine milieus) through artificial modeling approaches.

Distributed under CC BY-SA 4.0, from Wiki, without modification.

The fallopian tube is essential for orchestrating gamete transport, fertilization events, and preimplantation embryogenesis. While oviductal epithelial cells (OECs) have been examined in monolayer cultures, these static 2D environments fail to retain critical functional attributes—such as coordinated intercellular communication and secretory specialization—observed in native tissue. Conventional planar culture methods inadequately replicate the polarized secretory dynamics and maturation processes inherent to OEC physiology.

Oviduct-on-a-chip

Our bioengineered fallopian tube platform enables dynamic hormone-responsive cultures through programmable fluidic control—specifically facilitating hormonal pulsatility emulation unattainable in traditional culture dishes. Key innovations include: 1) Tissue-specific spatial confinement replicating in vivo cellular niches; 2) Spatiotemporal regulation of biomechanical/ biochemical parameters; 3) Gradient-enabled analysis of directed motility and phenotypic specialization. The microfluidic system's miniaturization optimizes rare cell utilization while enhancing resolution in high-throughput screening through concentrated microvolumes (vs. mL-scale systems). Integrated real-time imaging captures proliferative and migratory behaviors, with future interoperability planned across reproductive MPS modules (ovary, endometrium) for multi-organ interaction studies.

• A dog oviduct-on-a-chip model of serous tubal intraepithelial carcinoma

Ranking fifth among gynecological cancer mortalities, ovarian malignancies exhibit a stark 47% five-year survival prognosis. High-grade serous carcinoma (HGSC)—the predominant epithelial subtype—arises from transformed secretory cells within the oviduct, progressing through serous tubal intraepithelial carcinoma (STIC) precursors. This investigation engineered an ex vivo STIC analog via 53 knockout in canine oviductal epithelia cultured within microfluidic bioreactors. The mutagenized chip recapitulated hallmark STIC pathophenotypes: epithelial depolarization, ciliary attrition, hyperproliferative stratification, and molecular signatures mirroring human disease (elevated Ki67/PAX8/Myc transcript levels; suppressed PTEN/RB1 activity). This translational platform enables mechanistic exploration of premalignant transformation and biomarker discovery for HGSC early detection.

Fig 2. Oviduct-on-a-chip model, leakage test, and epithelial cell culture.^1,3

• An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Over five million infants globally have been conceived via assisted reproductive technologies (ART), yet animal studies indicate that in vitro embryo culture adversely impacts offspring (epi)genomic stability. During natural conception, the oviduct facilitates critical embryonic (epi)genetic reprogramming prior to uterine implantation. To investigate these mechanisms, scientists developed an oviduct-on-chip platform that replicates physiological reprogramming dynamics more faithfully than conventional IVF. This microfluidic system preserves zygotic (epi)genomic integrity by emulating in vivo conditions, aiding the identification of factors essential for fertilization and blastocyst quality.

Fig 3. Oviduct-on-a-chip platform—design and fabrication.^2,3

Applications

Oviduct-on-a-chip models have a wide range of applications in reproductive biology and related fields, including:

• Fertilization Studies: Investigating the mechanisms of sperm-oocyte interaction and the factors influencing fertilization success.
• Embryo Development Research: Studying the effects of the oviductal environment on early embryo development, including the role of oviductal fluid, hormones, and cell-cell interactions.
• Reproductive Disease Modeling: Modeling oviductal pathologies, such as salpingitis and endometriosis, to understand their pathogenesis and develop new treatments.
• Drug Discovery and Toxicology: Evaluating the effects of drugs and toxins on oviductal function and early embryo development.
• Assisted Reproductive Technologies (ART): Optimizing in vitro fertilization (IVF) procedures and improving embryo culture conditions.

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Why Choose Us?

Creative Biolabs is committed to providing our clients with cutting-edge oviduct-on-a-chip solutions that accelerate their research and drive innovation in reproductive biology. We offer several key advantages:

• Customization: We tailor our oviduct-on-a-chip systems to your specific research needs, ensuring optimal performance and relevance to your experimental design.
• Expertise: Our team comprises experienced scientists and engineers with extensive knowledge in microfluidics, cell biology, and reproductive biology.
• Advanced Technology: We utilize state-of-the-art microfabrication techniques and cell culture methods to create highly functional and physiologically relevant oviduct-on-a-chip systems.
• Comprehensive Support: We provide comprehensive support throughout the project, from initial consultation to final data analysis, ensuring your success.

FAQs

Recommended Products

For researchers facing challenges in initiating microfluidic cellular investigations de novo, Creative Biolabs' engineered cell-culture systems deliver integrated solutions that streamline workflow bottlenecks.

Our chips offer the freedom to choose the cell seeding channels and perfusion conditions, enabling various cell culture modes.

For more information about Creative Biolabs products and services, please contact us.

References

1. de Almeida Monteiro Melo Ferraz, Marcia, et al. "A dog oviduct-on-a-chip model of serous tubal intraepithelial carcinoma." Scientific Reports 10.1 (2020): 1575.
2. Ferraz, Marcia AMM, et al. "An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming." Nature Communications 9.1 (2018): 4934.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.