Single Cell Sequencing

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As a pioneer and the undisputed global leader in the field of microfluidic chips, Creative Biolabs offers a suite of custom microfluidic chip development services to support your single-cell sequencing projects.

Introduction of Single-Cell Sequencing

As the basic units of biological structure and function, cells vary largely in both type and state. The understanding of the functional capacities and responses of each cell type would accelerate the development of biology to a large extent. As a blueprint of cellular identity and function, the genome contains the entirety of an organism’s information. The primary challenge for genomes study at the single-cell level is the low quantities of DNA available, as well as the limitations in experimental throughput. In this case, it is necessary to find an easy enough way to analyze a large number of individual cells simultaneously.

Microfluidic Chips Development for SiC-seq

Here, we describe a novel approach for single-cell genome sequencing (SiC-seq) using droplet microfluidics. According to the isolation, amplification, and coding of single-cell genomes, the genomes of tens of thousands of cells can be sequenced in a single experiment. In this system, the cells are encapsulated in micron-scale hydrogels and lysed, segmented individually. And then combined with a microdroplet containing a unique oligonucleotide barcode, according to a single overlap extension polymerase chain reaction (PCR), the oligonucleotide barcode is spliced onto the cell's genomic DNA. Finally, a barcoded library (>50,000 single cells) ready for sequencing can be constructed in a matter of hours. With the advantages of high-throughput and low-bias, the SiC-seq enables a broader range of genomic studies at diverse cell populations.

Overview of the SiC-seq workflow. Fig.1 Overview of the SiC-seq workflow. (Demaree, B 2018)

Microfluidic Chips Development for RNA-seq

As a powerful tool for quantifying gene expression heterogeneity, the single-cell whole-transcriptome analysis has been widely used in multiple biological processes. Based on the microfluidic platform, the single-cell RNA-seq presents promise sensitivity. In this system, single cells are captured and lysed in a microfluidic device, and then the mRNAs with poly(A) tails are reverse-transcribed into cDNA. Finally, the double-stranded cDNA can be collected and sequenced with the next-generation sequencing technology. In conclusion, the combination of microfluidic technology and high-throughput sequencing provides great advantages for single-cell transcriptome analysis.

Device schematic and experimental pipeline. Fig.2 Device schematic and experimental pipeline. (Streets, 2014)

Based on our advanced platforms, we can provide microfluidic chips with the highest quality standards. We can also offer custom solutions in case our standard chips do not meet your requirements. If you are interested in our services, please do not hesitate to contact us for more detailed information. We are always pleased to help customers evaluate microfluidics concepts quickly and easily.

References

  1. Demaree, B.; et al. An ultrahigh-throughput microfluidic platform for single-cell genome sequencing. Journal of visualized experiments: JoVE. 2018 (135).
  2. Streets, A.; et al. Microfluidic single-cell whole-transcriptome sequencing. Proceedings of the National Academy of Sciences. 2014, 111(19): 7048-7053.

For Research Use Only. Not For Clinical Use.

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