Single-cell Sequencing

Inquiry

A cell is a basic unit of biological structure and function, varying broadly in type and state. The characterization of single-cell identity and function will accelerate biological discovery and development, as the understanding of functional abilities and responses of each cell type. As a first-class provider in the microfluidic chip market, Creative Biolabs has presented a reliable technology platform to support single-cell sequencing services and to analyze a large number of individual cells simultaneously. Aided by our sophisticated engineers, a number of sequencing strategies, such as based on droplet microfluidics, have been introduced to characterize complex tissues with varieties of cell lines and meet the demand of rapid, scalable approaches.

Single Cell Sequencing

Next-Generation sequencing (NGS) is a technology enabling the sequencing of the entire human genome within one day. Firstly, the entire genome is divided into millions of fragments and sequenced in parallel. Next, data collected from individual fragments are mapped to a reference human genome through bioinformatics analysis. The process is repeated many times to obtain accurate information on any DNA mutation. As well, NGS can be used for extracting data from desired genetic locations and has better sensitivity towards low-frequency variants because of its high sequencing depth.

NGS along with microfluidic technique has multiple applications in DNA sequencing, RNA sequencing, and ATAC sequencing (chromatin) of many kinds of individual cells. Some of the major technologies have been developed recently, especially single-cell RNA-Seq that displays quantitatively comparable to multiplexed quantitative PCR (qPCR).

Microfluidic diffusion-based RNA-seq (MID-RNA-seq) device and operation. Fig.1 Microfluidic diffusion-based RNA-seq (MID-RNA-seq) device and operation. (Sarma, 2019)

Microfluidic Platform & Service at Creative Biolabs

The ability to associate single-cell genetic information to cellular phenotypes could present a detailed insight into human physiology and disease mechanisms that is hard to infer from bulk cell analysis. Microfluidic technologies are valuable and attractive for single-cell manipulation owing to strict handling and low risk of contamination. Incorporating microfluidic platforms into research and clinical workflows will fit an unmet need in biology, depicting the highly precise and informative results necessary to develop novel therapies and monitor patient outcomes. At Creative Biolabs, we’d like to introduce the following strategies to perform single-cell sequencing services in terms of microfluidic technologies.

Single-cell transcriptome sequencing sensitivity. Fig.2 Single-cell transcriptome sequencing sensitivity. (Street, 2014)

Key-benefits

Advantages of microfluidics for single-cell genetic analysis. Fig.3 Advantages of microfluidics for single-cell genetic analysis. (Thompson, 2014)

High-throughput, microfluidic-based single-cell sequencing is rapidly stepping forward with technical upgrades and additional software to extend the scientific understanding of genomics studies at the level of single cell and single nucleus. As a well-recognized expert in the microfluidic field, Creative Biolabs has developed a series of advanced platforms and personalized strategies to accomplish single-cell sequencing services. We always pay attention to the current and future potential utilization of microfluidics at all stages of single-cell genetic analysis, including cell enrichment and capture, single-cell compartmentalization, and manipulation, as well as detection and sequencing analyses. If you’ like to know more about our custom microfluidic chips, please directly contact us or send us an e-mail with your requests.

References

  1. Sarma, M.; et al. A diffusion-based microfluidic device for single-cell RNA-seq. Lab Chip. 2019, 19(7): 1247-1256.
  2. Street, A.M.; et al. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci USA. 2014, 111(19): 7048-7053.
  3. Thompson, A.M.; et al. Microfluidics for single-cell genetic analysis. Lab Chip. 2014, 14(17): 3135-3142.

For Research Use Only. Not For Clinical Use.

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