Evaluate Cell Growth

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Creative Biolabs is one of the well-recognized experts who are professional in applying advanced microfluidic chip technologies for a broad range of project objectives. We offer custom solutions to meet the special needs of our clients all over the world.

Introduction of Microfluidic-based Cell Culture

The novel microfluidic technology provides well-controlled parameters and micro-scale complex structures, which benefit to mimic the in vivo environment of cells. The combination of microfluidic technology with 3D cell culture provides great potential in a variety of applications, such as drug activity evaluation and high-quantity mAb production.

Microfluidic Chips for Drug Activity Evaluation

In recent years, cancer has been a leading cause of death worldwide and there is an understandable need for cancer therapy development. Tumor Spheroid (TS) is considered the best cellular model for cancer research. As tumor growth rate and response to anticancer drugs are largely determined by tissue structure, the three-dimensional tumor cell models are more competitive than two-dimensional ones. Here, we describe a three-dimensional microfluidic chip for tumor spheroid formation, cultivation, and analysis. By introducing the drugs that inhibit cell growth into the microsystem at different frequencies and different concentrations, the slowing and stopping of cell growth can be observed to evaluate the effects of the drugs. Compared with other viability assays that require termination of a culture, it is suitable for long-term observation of spheroid response.

Geometry and fabrication of the device. Fig.1 Geometry and fabrication of the device. (Ziółkowska, K 2013)

Cell Growth Evaluation for mAb Production

In recent decades, therapeutic antibodies present great potentials for the treatment of multiple diseases. In order to obtain high-quantity therapeutic recombinant proteins, it is necessary to optimize the growth of expressing cells. Microfluidic chips are novel cell culture devices that allow cell growth under highly controlled conditions. In contrast to traditional approaches, the microfluidic-based cell culture can maintain well-defined cell culture conditions, and also provide cells with fresh media containing oxygen, nutrients, and carbon dioxide continuously. Here, we evaluated HEK-293T cell growth under different microfluidic experimental conditions. The results showed that cells cannot attach on any surface of the microdevice channel in absence of cell adhesion molecules, while cells were attached to the bottom of the microchannel when cell adhesion molecules were present. Besides, different microchannel geometries and thicknesses, as well as the influence of the flow rate showed great influence in cell adhesion and growth.

Microfluidic chip designs for mAb production. Fig.2 Microfluidic chip designs for mAb production. (Peñaherrera, 2016)

With years of experience, our scientists have developed various microfluidic chips with the highest quality standards for different purposes. We can also offer custom solutions in case our standard chips do not meet your requirements. If you are interested in our services, please do not hesitate to contact us for more detailed information.

References

  1. Ziółkowska, K.; et al. Long-term three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip. Biosensors and Bioelectronics. 2013, 40(1): 68-74.
  2. Peñaherrera, A.; et al. Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production. Microelectronic Engineering. 2016, 158: 126-129.

For Research Use Only. Not For Clinical Use.

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