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With a variety of advantages, the advent of microfluidic chips has brought cell separation and sorting more possibilities. With years of experience focusing on microfluidic chip development, Creative Biolabs is capable of offering the highest standard microfluidic chip to help our customers tackling difficulties in cell separation and sorting.
Microfluidic technologies for cell separation in the biomedical field are booming in the recent 15 years. Benefitted from the microscale features approaching a single cell, microfluidic cell sorting devices have shown many attractive advantages compared to their forerunners (e.g., fluorescence-assisted cell sorting (FACS) and magnetic-assisted cell sorting (MACS)). With the advance in diagnostic and therapeutic medicine for point-of-care tests, the need for cell separation has been greatly expanded nowadays. In this context, microfluidic-based cell separation and cell sorting technologies have shown excellent performance.
Microfluidic devices capable of measuring cellular biophysical properties can also prove useful for cancer cell detection. A few microfluidic devices have been developed to measure single-cell mechanical and/or electrical properties, enabling the discrimination of normal cells from malignant counterparts. For example, researchers developed a microfluidic optical stretcher for cancer cell mechanical characterization, indicating that cells with higher metastatic potentials (e.g., Mod-MCF-7) deformed more than normal cells (e.g., MCF-10). Furthermore, another group reported a microfluidic system for cell-type classification using both mechanical and electrical parameters of cells, demonstrating that electrical and mechanical parameters, when used in combination, can provide a higher cell classification success rate in distinguishing EMT6 (murine breast cancer cell lines).
Fig. 2 DLD cell sorting.1
Magnetic activated cell sorting relies on the interaction between cell surface antigens and antibodies conjugated to suspended magnetic particles. Magnetic bead-based techniques readily permit the manipulation of captured cancer cells using local magnetic fields. Multi-functional, integrated microfluidic devices capable of cancer cell separation, cell lysis, and genetic identification were reported. This platform consisted of an incubation module where target cancer cells are selectively captured onto functionalized magnetic beads, a control module for sample transportation, and a nucleic acid amplification module for cell lysis and genetic identification. Cancer cells (e.g., lung and ovarian carcinoma) were spiked into whole blood samples and loaded into the incubation chamber with pre-loaded magnetic beads coated with monoclonal antibodies. The cancer cells were specifically immobilized onto the surface of the magnetic beads with a recovery rate higher than 90%. The purified magnetic complexes were subsequently resuspended and transported to the cell lysis/reverse transcription chamber where the expressed genes associated with ovarian and lung cancer cells were successfully amplified.
Creative Biolabs has developed a comprehensive technology platform that is dedicated to developing and designing the microfluidic chip. We are confident in providing our customers with the highest standard microfluidic chip. We also provide microfluidic chip development services to meet customers’ different requirements. If you are looking for a better solution for cell sorting or separation, please don’t hesitate to contact us for more information.
The microfluidic chips enable precise sorting of cells based on various physical and biochemical properties, such as size, shape, and dielectric properties. This precision ensures that the separated cell populations are highly pure and suitable for downstream applications.
These microfluidic devices require minimal sample volumes, often in the nanoliter range. This feature is particularly beneficial when working with scarce or precious samples, such as patient-derived cells or rare cell populations.
The gentle handling of cells within the microfluidic channels minimizes stress and potential damage to the cells. This is critical for maintaining cell viability and functionality, especially for sensitive cell types.
Our chips offer label-free cell separation, relying on intrinsic cell properties rather than external labels or markers. This approach reduces preparation time and avoids potential interference from labeling agents.
Our microfluidic chips are highly customizable to meet specific research needs. Whether it's adjusting channel dimensions or incorporating different sorting mechanisms, we can tailor the chips to optimize performance for various applications.
Reference
For Research Use Only. Not For Clinical Use.