Creative Biolabs' Microfluidic Chip Development Service provides bespoke solutions for integrating and optimizing Polymerase Chain Reaction (PCR) within microfluidic platforms. Our service delivers highly efficient, sensitive, and cost-effective PCR systems tailored to your specific research or diagnostic needs. You can expect miniaturized reaction volumes, significantly reduced assay times, and enhanced automation, leading to higher throughput and reduced manual intervention. We empower you to achieve precise genetic analysis, rapid pathogen detection, and advanced genomics with unparalleled efficiency.
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Background
Microfluidics, the discipline governing fluid behavior at micrometer dimensions, has transformed molecular biology, specifically in polymerase chain reaction (PCR) implementations. PCR, a cornerstone technique for amplifying specific DNA sequences, benefits immensely from miniaturization on microfluidic chips, leading to faster reaction times, reduced reagent consumption, and enhanced automation. This integration forms the basis of microfluidic PCR, a powerful tool for diverse applications from diagnostics to basic research.
PCR's essential thermal phases—denaturation, annealing, and extension—are accurately managed within micro-conduits and reaction chambers. Microfluidic systems inherently provide analytical method miniaturization alongside operational and manufacturing simplicity, accelerated processing, and diminished reagent utilization per assay for nucleic acid isolation and amplification. Chip miniaturization not only increases amplification velocity but also achieves orders-of-magnitude PCR volume reduction. Lower Taq polymerase usage potentially slashes per-test expenses substantially. Correspondingly, reduced reagent quantities decrease hazardous waste disposal requirements while eliminating contamination risks and procedural errors from additional experimental stages. Moreover, these systems enable integrated execution of multiple processes—from DNA extraction through subsequent analytical stages (PCR and electrophoretic separation)—on a single microdevice.
Fig. 1 Microfluidic cell encapsulation and microfluidic PCR.1,4
A proven microfluidic PCR advancement involves a high-capacity platform for flow-through amplification within nanoscale water-in-oil droplets. The chip's circular architecture enables droplet traversal through distinct thermal zones, completing 34 PCR cycles within minutes while eliminating full-device thermocycling. This system integrates multiple microfluidic innovations with minimal volumes to ensure elevated reaction efficiency alongside reduced reagent and sample usage. Temperatures for the implemented protocol are adjustable; product analysis confirms amplification specificity equivalent to conventional PCR instruments. The heightened efficiency permits single-DNA-molecule amplification per droplet, enabling robust, reproducible experiments across diverse applications.
Applications for Microfluidic Chip Development Service for Polymerase Chain Reaction
The versatility and efficiency of microfluidic PCR chips open doors to a wide array of applications across various sectors:
Clinical Diagnostics
Rapid, accurate detection of pathogens, genetic mutations, and disease biomarkers for infectious diseases, oncology, and inherited disorders, benefiting point-of-care testing.
Oncology Research
High-throughput DNA genotyping to study tumor heterogeneity, clonal evolution, and drug resistance, offering insights for personalized medicine.
Forensic Science
Automated, contamination-free identification of biological samples (e.g., animal family identification, DNA profiling) from trace evidence, enhancing investigation speed and reliability.
Environmental Monitoring
Rapid detection of contaminants and pathogens in environmental samples, and genetic analysis of microbial communities.
Food Safety
Fast, reliable detection of foodborne pathogens, allergens, and adulterants, ensuring consumer safety.
Agricultural Biotechnology
Genetic screening of crops and livestock for desirable traits and disease resistance, accelerating breeding and improving yields.
Basic Research
Fundamental studies in molecular biology, genomics, and epigenetics, enabling complex assays with minimal resources for faster discoveries.
What We Can Offer
Creative Biolabs offers a comprehensive suite of products and services designed to meet your specific microfluidic PCR needs:
Leverage our specialized benefits—Request a quotation today
Workflow
Why Choose Us
Creative Biolabs stands at the forefront of microfluidic innovation, offering unparalleled expertise and cutting-edge solutions for PCR chip development. Our dedication to accuracy, efficacy, and customer outcomes differentiates our offerings.
Key Advantages:
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Enhanced PCR Performance:
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Cost-Effectiveness
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Customization and Integration
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Expertise in Novel Technologies
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Automation and Throughput
Published Data
Research findings from studies on microfluidic chip-based PCR systems are detailed:
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Centrifugally-Driven Nested PCR Disc System
Fig 2. Schematic description of a microfluidic disk segment.2,4
Researchers led by Mark Keller engineered an automated microfluidic disk for nested real-time PCR, designed to meet forensic criteria and facilitate the identification of prevalent European animal species. This PCR variant employs two primer sets, rather than a single pair, to amplify a target DNA segment, thereby enhancing both its specificity and sensitivity over conventional PCR methods. Each microfluidic disk incorporates four distinct segments. One such segment integrates a preamplification chamber for samples and no-template controls (NTC), a capillary siphon valve for product transfer, and a centrifugal thermopneumatic two-stage dispenser for 14 sample and one NTC main amplifications. Experimental outcomes demonstrated fundamental concordance with reference sequencing, alongside improved resolution. The system's pre-stored reagents within GeneSlice and its sealed reaction environment eliminate the need for additional manual manipulation, consequently decreasing processing time and contamination hazards. Its functional reliability has been validated at up to 92.2% across various samples.
FAQs
Q: How do microfluidic PCR systems contribute to cost reduction in molecular assays?
A: Microfluidic chips significantly reduce reaction volumes, leading to substantial decreases in reagent consumption. Additionally, their integrated and automated nature minimizes hands-on time and equipment needs, contributing to overall operational savings.
Q: To what extent can microfluidic chips be tailored for specific assay requirements?
A: Microfluidic chips offer extensive customization. Designs can be precisely tailored for unique assays, including specific target detection, multiplexing, or integrating upstream sample preparation steps, ensuring optimal performance for diverse research and diagnostic needs.
Q: What level of analytical sensitivity can be achieved with microfluidic PCR compared to conventional methods?
A: Microfluidic PCR, particularly droplet-based digital PCR (ddPCR) on these platforms, offers exceptional sensitivity, capable of detecting extremely low concentrations of target DNA, even down to single copies. This enhanced sensitivity is crucial for precise quantification or detection of rare events.
Featured Services
Feature Products
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CAT No
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Material
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Product Name
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Application
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MFCH-001
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Glass
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Herringbone Microfluidic Chip
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Processing samples and reagents in Nucleic acid analysis, blood Analysis, immunoassays and point-of-care diagnostics.
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MFMM-0723-JS12
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Glass
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Double Emulsion Droplet Chip
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Our double emulsion microfluidic chip, incorporating localized modifications and a classic flow-focusing structure, is specifically designed to generate stable and uniform double emulsion droplets.
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MFCH-005
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PDMS
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3D Cell Culture Chip-Neuron
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Neuron cell culture and study of axon transport, axon protein synthesis, axon damage/regeneration, signal transduction of axon to somatic signal.
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MFCH-009
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PDMS
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Synvivo-Idealized Co-Culture Network Chips (IMN2 radial)
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SynBBB 3D Blood Brain Barrier Model/SynRAM 3D Inflammation Model/SynTumor 3D Cancer Model/SynTox 3D Toxicology Model
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MFMM1-GJS4
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COC
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BE-Doubleflow Standard
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Studying circulating particles, cell interactions and simple organ on chip system construction.
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MFMM1-GJS6
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COC
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BE-Transflow Custom
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Used to construct cell interface or Air-Liquid interface (ALI) to study more complex culture systems.
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Click here to Explore our complete product catalog.
For detailed inquiries regarding our offerings, reach out to our specialists.
References
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Pellegrino, Sciambi, et al. " High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics." Genome Research 28 (2018): 1345-1352. https://doi.org/10.1101/gr.232272.117
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Keller, Mark, et al. "Automated forensic animal family identification by nested PCR and melt curve analysis on an off-the-shelf thermocycler augmented with a centrifugal microfluidic disk segment." PloS one 10.7 (2015): e0131845. https://doi.org/10.1371/journal.pone.0131845
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Fergola, Andrea et al. "Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies." Biosensors vol. 15,6 345. 29 May. 2025, https://doi.org/10.3390/bios15060345
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Distributed under Open Access license CC BY 4.0, without modification.
