Microfluidic Chip Development Service for Polymerase Chain Reaction
Inquiry
The microfluidic chips hold promise for convenient integration and have the ability to integrate a Polymerase Chain Reaction (PCR) module to add a critical missing component to the lab-on-a-chip (LOC) toolkit. As a leader in microfluidic chips development, Creative Biolabs has rich experience and an efficient microfluidic technology platform to provide high-quality services related to microfluidic chip design and development for our global customers.
Why we perform PCR on Microfluidic Chips?
The typical method for detecting trace amounts of nucleic acid is to amplify target DNA to a certain amount by using PCR for DNA amplification, followed by quantitative detection. The PCR is the most classical technique for DNA amplification customarily carried out in laboratory-scale PCR cycles with applications ranging from diagnostics, cloning, to sequencing. Nowadays, it is possible to miniaturize the process in microfluidic chips, reducing the cost of fabrication and consumption of biological samples, and also the time of DNA amplification. Moreover, chip-based microfluidic systems are amenable to integrate with other DNA processing and analysis steps. Thus, the integration of microfluidic chips with PCR-based amplification can be broadly used for genetic analysis in the fields of molecular biology and clinical diagnosis to achieve ultrasensitive and rapid detection of trace amounts of nucleic acids.
The benefits of the microfluidic system inherently scale reduction to the analytical method and also have simplicity in function and fabrication, rapid turnaround time, decreased reagent consumption per test for DNA extraction and amplification. The reduced size of microfluidic chips not only enhances amplification speed but also provides dozens of reduction in PCR volume. Consuming less Taq polymerase yields the potential to decrease the cost per test dramatically. Concordantly, the decrease in the number of reagents reduces the hazardous waste that must be disposed of and eliminates possible contamination and error during the extra experimental steps. Furthermore, the system could also display the integration of many reactions from DNA extraction to multiple downstream processes (PCR and electrophoretic analysis) on the same microdevice.
Fig. 1 Microfluidic cell encapsulation and microfluidic PCR.1,3
How to perform PCR on Microfluidic Chips?
A successful example of microfluidic chips development for PCR is a high throughput microfluidic device for continuous-flow PCR in water-in-oil droplets of nanoliter volumes. The circular design of the chip allows droplets to pass through alternating temperature and complete 34 cycles of PCR in only a few minutes, avoiding the temperature cycling of the entire device. This system brings together many advances in microfluidics with a small volume to ensure high reaction efficiency and low reagent and sample consumption. The temperatures for the applied temperature PCR protocol can be adjusted; analysis of the product shows that the amplification is specific comparable to a benchtop PCR machine. The high efficiency allows amplification from a single molecule of DNA per droplet to carry robust and reproducible PCR experiments with high efficiency in several potential applications.
Based on an advanced microfluidic technology platform, Creative Biolabs provides the design and development of the microfluidic chips for PCR solutions on-chip, DNA purification, and PCR-based amplification, followed by separation and detection in a manner to create an integrated system with a level of control on the macroscopic scale. Meanwhile, our analytical platform for PCR technology utilizes cost-effective microfluidic chips to reduce reagent consumption by orders of magnitude, provide turnaround times, and offer the potential of rapid, inexpensive on-site screening. It is reasonable to expect that the small microfluidic chips can be assembled around the portable instrumentation to generate a portable handheld system, which will be applicable in several different clinical contexts.
Microfluidic PCR devices provide sample-in and answer-out genetic testing and present substantial opportunities to reduce the cost, size, and energy requirements of diagnostic tools. Along with over years of extensive experience in developing microfluidic chips, scientists at Creative Biolabs are proud to tailor the best-fit microfluidic chips development solutions for PCR to meet our customers' specific project requirements. Please feel free to contact us for more information.
Published Data
Presented are findings showcased within articles pertaining to PCR on microfluidic chip:
1. Nested PCR with a Centrifugal Microfluidic Disk Segment
Fig. 2 Schematic description of a microfluidic disk segment.2,3
Mark Keller et al. developed a microfluidic disk that enables automated nested real-time PCR testing. This test is suitable for forensic standards and can be used to identify common European animal groups. Nested PCR is a variant of the polymerase chain reaction (PCR) that uses two pairs of PCR primers instead of one to amplify the entire fragment. It offers greater specificity and sensitivity compared to standard PCR. A microfluidic disk segment contains four microfluidic disk segments. One segment includes a preamplification chamber for sample and no template control (NTC) liquid, a capillary siphon valve for transferring preamplification products, and a centrifugal the rmopneumatic two-stage dispensing structure for 14 (sample) and 1 (NTC) main amplifications respectively. The experimental results show a basic match with the reference sequencing reaction and show better resolution. The pre-storage of reagents inside GeneSlice and its closed reaction environment do not require further manual handling, reducing the time required for handling and the risk of contamination. The reliability of this function has been proven to be up to 92.2% in samples.
References
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Pellegrino, Sciambi, et al. " High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics." Genome Research 28 (2018): 1345-1352.
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Keller, Mark, et al. "Automated forensic animal family identification by nested PCR and melt curve analysis on an off-the-shelf thermocycler augmented with a centrifugal microfluidic disk segment." PloS one 10.7 (2015): e0131845.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not For Clinical Use.
