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As a well-established company in the bio-industry, Creative Biolabs offers a full range of microfluidic chip development services. Guaranteed to perform within the specified time frame, and the quality of microfluidic chip research is always maintained to the end. Our laboratory services have many advanced psychiatric in vitro diagnostic tools, such as DNA hybridization, to support customer’s needs in the fast-growing point-of-care technology (POCT) diagnostic discovery projects.

Introduction to DNA Hybridization

Hybridization is a type of new technology for combining two complementary deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules that are paired by bases to form a single-stranded double-stranded molecule. Normally, DNA hybridization techniques can be classified into two main types, DNA-DNA hybridization and DNA-RNA hybridization, according to the type of nucleic acid bound. Double-stranded DNA (ds) DNA are usually stable in the laboratory. However, it can separate into single strands while increasing ambient temperature. These chains complement each other, but can also be complementary to other sequences around them. Moreover, single-chain molecules can also be annealed or "hybridized" with each other by lowering the ambient temperature.

In recent years, DNA hybridization has been considered to be a powerful tool for the diagnosis of various diseases, such as infectious diseases. This technology can detect many kinds of samples, such as tissue or body fluids. DNA probes used for hybridization analysis can detect extremely small amounts of homologous DNA sequences. Under the right conditions, these DNA probes are reliable and accurate for the pathogen or gene being tested. Up to now, radioactivity has been broadly used as a novel detection probe for DNA hybridization, but it still takes a lot of time to get the diagnostic data. Therefore, New probes for additional microbial genes are being developed and refined, and they may allow DNA hybridization to play an increasing role in diagnostic pathology.

Fig. 1 Nucleic acid hybridization.^1,2

Microfluidic Chip Development for DNA Hybridization

DNA hybridization has become a standard technology applied in diagnostic assays. As a new diagnostic tool, microfluidic chip has shown its promising role in detecting disease samples for a prompt on-site diagnosis and treatment. Recent reports have indicated that a panel of microfluidic DNA hybridization assays has been generated for providing rapid, high sensitivity, high hybridization kinetics, as well as high specific target-probe binding detection.

Equipped with a team of professional scientists, we have established a variety of microfluidic DNA hybridization assays for our clients for drug analysis. For example, two DNA hybridization microfluidic chips have been developed by our labs for directly delivering micro samples into a DNA hybrid array, and allowing hybridization, washing, or scanning procedures to be performed simultaneously. These chips can also rapidly achieve probe saturation levels intending to transport fixed detection sites for quantitative analysis of samples. Besides, we have successfully developed a microfluidic technology that combines polymerase chain reaction (PCR) and DNA hybridization for analyzing different bacterial pathogens. In general, a battery of universal primers of PCR will be designed and the PCR product will be introduced into a microarray hybrid chip containing a fixed DNA probe. These data have suggested that it is a promising platform for clinical bacterial identification that can be further applied to point-of-care diagnosis.

Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in the field of microfluidic chip development with our customers. We offer high-quality customized services to develop well-validated and highly published microfluidic chip-based DNA hybridization analysis technologies with your disease-of-interest. We are building a team of talented and motivated scientists and technicians to pursue our commitment and have won a good reputation among our worldwide customers for accomplishing numerous challenging projects in this field. For more information, please feel free to contact us or send us an inquiry.

References

1. Fusco, Vincenzina, and Grazia Marina Quero. "Nucleic acid-based methods to identify, detect and type pathogenic bacteria occurring in milk and dairy products." Structure and function of food engineering (2012): 371e404.
2. under Open Access license CC BY 3.0, without modification.

For Research Use Only. Not For Clinical Use.