Based on our advanced microfluidic platform, Creative Biolabs offers the design and development services of microfluidic chips in order to realize the high-efficient and low-cost manufacturing of microfluidic chips for the detection of nucleic acids. It provides a simple and high throughput analysis method for rapid nucleic acids detection to meet the specific needs of customers.
Direct detection of nucleic acids on microfluidic chips offers a convenient method for gene detection and analysis without a series of complicated reactions. These approaches are similar to the specific recognition of antibody to antigen. Based on the complementarity of the nucleotide sequence, nucleic acids can be directly detected and measured on microfluidic chips. The system can also be used for the simultaneous detection of multiplex nucleic acids based on a sandwich-like DNA hybridization reaction with a pair of probes for target DNA. The precise control and process of small volumes of samples with high efficiency and speed can be achieved with microfluidics technology. It also holds great promise to develop simple, ultrasensitive, highly selective, and cost-effective biosensors.
Recently, some researchers have employed several multiplexed barcoded paper-based assay (BPA) on chips for the analysis of nucleic acids. Target DNAs can recognition with capture DNAs and detection DNAs via base complementation, in which the capture DNA is immobilized on paper-based detection microfluidic chip, and the detection DNA is modified with gold nanoparticles (AuNPs). The target DNA in the sample solution hybridizes with the detection DNA on the surface of AuNPs; they are formed as target-detection DNA-AuNP complexes further to hybridize with the capture DNAs. Due to the color change in the detection zone, the concentration of the target DNA can be quantitatively read.
Fig.1 Multiplexed barcoded paper-based assay. (Yang, 2017)
Another example of a novel microfluidic biosensor for fluorescent DNA detection is by using single-layered MoS2 nanosheets as nanoprobes. This system takes advantage of different adsorption capacities of MoS2 nanosheets toward single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) to detect target DNA. The multi-channel is designed for rapid and sensitive high throughput DNA detection within several minutes. Besides, the PDMS-based microfluidic device with zigzag-shaped microchannels is designed for uniform mixing of various samples. Strong fluorescence intensity can be observed in microfluidic channels upon the addition of target ssDNA to the microfluidic chip. Compared to the fluorescence intensity before the addition of ssDNA, it enables the rapid, visual, and quantitative detection of target DNA on the microfluidic chip.
Fig.2 Microfluidic biosensor for fluorescent DNA detection. (Huang, 2015)
These examples confirm that the use of microfluidics can significantly reduce the nucleic acids sample volume and detect nucleic acids visibly within a few minutes through the microfluidic assay. All these detections on chips are simple, rapid, low-cost, robust, and even possible compatible with mobile devices; the whole detection process and the readout of the results can be completed within several minutes. Because of the high rapidity, simplicity, efficiency and selectivity, these microfluidic chips developed are suitable to be applied in the rapid and on-site detection of nucleic acids.
Creative Biolabs focuses on the development of highly sensitive and direct detection of nucleic acids in the context of microfluidic chips in the condition of enough concentration of nucleic acid in samples. Our advanced microfluidic technology platform can be used to realize the design of microfluidic chips with high precision and efficiency at a very low cost. If you are interested in our customized services about microfluidic chips, please do not hesitate to contact us for more information.
For Research Use Only. Not For Clinical Use.