Creative Biolabs' Microfluidic Chip Development Service for Nucleic Acid Detection delivers unparalleled precision, speed, and integration for your molecular diagnostic and research needs. We provide customized microfluidic solutions designed to overcome the limitations of traditional methods, enabling rapid, high-throughput, and cost-effective nucleic acid analysis. Our service focuses on creating integrated "sample-to-answer" platforms that streamline complex workflows, from sample preparation to detection, all on a single chip.
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Background
Traditional nucleic acid testing (NAT), exemplified by quantitative real-time polymerase chain reaction (qRT-PCR), while highly accurate, often suffers from limitations such as lengthy multi-step processes, high reagent consumption, and the need for specialized laboratory infrastructure and trained personnel. These challenges hinder rapid, large-scale, and decentralized diagnostic capabilities, as highlighted during recent global health crises.
Fig. 1 Nucleic acid detection by microfluidic.1,5
Microfluidic chip development directly addresses these pain points by integrating the entire NAT workflow—from sample preparation (cell lysis and nucleic acid purification) and amplification (PCR or isothermal methods like LAMP) to sophisticated detection (fluorescent, optical, electrochemical, or lateral flow assays)—onto a single, compact platform. This integration not only accelerates the testing procedure but also minimizes contamination risks and simplifies operation.
Beyond basic detection, microfluidic chips facilitate multiplexed analysis, allowing for the simultaneous detection of multiple genetic targets from a single sample. Innovations such as paper-based assays and the incorporation of nanomaterials like MoS2 nanosheets further enhance sensitivity, reduce costs, and enable highly portable solutions. The continuous-flow PCR (CF-PCR) integrated with electrophoresis on a microfluidic chip represents a significant advancement, offering rapid DNA amplification and detection critical for integrated bedside diagnostic platforms. This convergence of miniaturization, automation, and enhanced analytical performance positions microfluidic chip development as a cornerstone for the next generation of nucleic acid diagnostics and research.
Fig. 2 Microfluidic cell encapsulation and microfluidic PCR.2,5
Applications for Microfluidic Chip Development Service for Nucleic Acid Detection
Microfluidic chip development for nucleic acid detection offers versatile and efficient applications across numerous critical sectors, transforming molecular analysis.
Point-of-Care (POC) Diagnostics
Enables rapid, accurate, and decentralized testing for infectious diseases (e.g., SARS-CoV-2) at the patient's side, eliminating centralized labs.
Clinical Diagnostics & Personalized Medicine
Facilitates high-throughput genetic screening for inherited diseases, cancer biomarkers, and pathogen identification, supporting tailored treatments and early detection.
Food Safety & Environmental Monitoring
Provides rapid detection of foodborne pathogens (e.g., Salmonella), allergens, contaminants, environmental pollutants, and airborne pathogens.
Drug Discovery & Development
Accelerates drug screening, toxicity testing, and ADME studies via "Organs-on-Chips" technology, mimicking human physiology to reduce time and cost.
Agriculture & Veterinary Diagnostics
Offers on-site detection of plant pathogens, animal diseases, and genetic traits in livestock, enhancing crop protection and agricultural productivity.
Forensics & Biodefense
Supports rapid identification of biological samples at crime scenes and swift detection of biothreat agents, crucial for investigations and emergency response.
Basic Research & Genomics
Enables single cell analysis, high-throughput DNA sequencing, and complex cellular assays in controlled microenvironments, advancing fundamental biological understanding.
What We Can Offer
Creative Biolabs delivers an integrated portfolio of solutions and offerings engineered to advance your microfluidic nucleic acid detection initiatives. Our offerings are tailored to meet diverse research, diagnostic, and commercial needs.
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Workflow
Why Choose Us
Creative Biolabs leads microfluidic innovation with unmatched proficiency and a demonstrated history in creating pioneering nucleic acid analysis systems. Our commitment to precision, efficiency, and client success sets us apart, ensuring your projects achieve their full potential.
Key Advantages:
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Integrated "Sample-to-Answer" Solutions
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Rapid & High-Throughput Capabilities
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Cost-Effective & Reagent-Saving
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Portability & Decentralized Testing
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Customization & Expertise
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Proven Performance
Published Data
Presented are findings showcased within articles pertaining to microfluidic nucleic acids detection:
1. Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip
Fig. 3 Schematic showing the CF-PCR-MCE chip.3,5
Bo Yang et al. designed and fabricated a microfluidic chip combining continuous-flow PCR (CF-PCR) and electrophoresis, which can realize DNA amplification and on-site detection of PCR products in a short time.3,4 The chip consists of two PDMS blocks, the upper part is a cross-shaped channel, and the lower part is a serpentine channel loop structure. The chip is placed on a temperature control unit. The article verified the system by detecting typical periodontal pathogens. The results showed that the system successfully amplified and detected P.g, T.d, and T.f, and had a significant advantage in speed. This integrated structural design provides a valuable reference for the realization of an integrated bedside diagnostic platform.
FAQs
Here are some common questions potential clients have about advanced microfluidic solutions for nucleic acid detection:
Q: What are the key advantages of microfluidic platforms over conventional nucleic acid testing methods?
A: Microfluidic solutions significantly enhance traditional methods by integrating multiple steps (sample preparation, amplification, detection) onto a single chip. This leads to dramatically reduced assay times, lower reagent consumption, minimal sample volume requirements, and increased portability, making complex molecular diagnostics faster, more cost-effective, and accessible outside traditional lab settings.
Q: Is it possible to customize microfluidic chips for specific biological applications?
A: Absolutely. Tailored design forms the foundation of sophisticated microfluidic development. Chips can be tailored to your exact specifications, whether for detecting specific viral strains, bacterial pathogens, genetic mutations, or biomarkers. Our team works closely with you to ensure the chip and assay are optimized for your unique target and application.
Q: Beyond infectious disease diagnostics, what are the broader applications of microfluidic nucleic acid detection?
A: Microfluidic nucleic acid detection has broad applicability beyond infectious diseases. It is highly effective in areas such as personalized medicine (e.g., cancer biomarker detection, pharmacogenomics), food safety (pathogen and allergen detection), environmental monitoring, drug discovery (e.g., "Organs-on-Chips" for drug screening), agriculture, and forensic analysis. The core technology is versatile enough to be adapted to any application requiring precise and efficient nucleic acid analysis.
Featured Services
Feature Products
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CAT No
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Material
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Product Name
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Application
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MFCH-001
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Glass
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Herringbone Microfluidic Chip
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Processing samples and reagents in Nucleic acid analysis, blood Analysis, immunoassays and point-of-care diagnostics.
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MFMM-0723-JS12
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Glass
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Double Emulsion Droplet Chip
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Our double emulsion microfluidic chip, incorporating localized modifications and a classic flow-focusing structure, is specifically designed to generate stable and uniform double emulsion droplets.
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MFCH-005
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PDMS
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3D Cell Culture Chip-Neuron
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Neuron cell culture and study of axon transport, axon protein synthesis, axon damage/regeneration, signal transduction of axon to somatic signal.
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MFCH-009
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PDMS
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Synvivo-Idealized Co-Culture Network Chips (IMN2 radial)
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SynBBB 3D Blood Brain Barrier Model/SynRAM 3D Inflammation Model/SynTumor 3D Cancer Model/SynTox 3D Toxicology Model
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MFMM1-GJS4
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COC
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BE-Doubleflow Standard
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Studying circulating particles, cell interactions and simple organ on chip system construction.
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MFMM1-GJS6
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COC
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BE-Transflow Custom
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Used to construct cell interface or Air-Liquid interface (ALI) to study more complex culture systems.
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Click here to Explore our complete product catalog.
For detailed inquiries regarding our offerings, reach out to our specialists.
References
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Mumtaz, Rashid, et al. " Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches." Biosensors 13.6 (2023): 584. https://doi.org/10.3390/bios13060584
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Pellegrino, Sciambi, et al. " High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics." Genome Research 28 (2018): 1345-1352. https://doi.org/10.1101/gr.232272.117
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Yang, Bo, et al. "Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip." Separations 10.4 (2023): 271. https://doi.org/10.3390/separations10040271
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Fergola, Andrea et al. "Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies." Biosensors vol. 15,6 345. 29 May. 2025, https://doi.org/10.3390/bios15060345
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Distributed under Open Access license CC BY 4.0, without modification.
