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Creative Biolabs is a leading service provider that focuses on microfluidic chip development. With years of experience, our scientists have developed a variety of microfluidic chips for multiple applications. Now we are pleased to provide high-quality microfluidic chip development services for the detection of genetic mutations for our clients all over the world.

Introduction of Genetic Mutations Detection

Genetic detection is a diverse and growing field with a series of applications, including diagnosis, prognosis, and treatment. As highly specific biomarkers to distinguish cancer cells from their normal counterparts, the detection of genetic mutations provides a wealth of diagnostic and prognostic information. The traditional techniques such as dual-probe TaqMan® assays and pyrosequencing can achieve quantitative detection. However, in a background of non-mutated DNA from normal cells, they cannot detect less than ~1% mutant genes. In recent years, the development of a novel microfluidic system allows the highly sensitive detection of mutated DNA in complex samples.

Fig.1 Overview of droplet-based microfluidic system. (Pekin, D 2011)

Microfluidic Chips Development for Detection of Genetic Mutations

In recent years, a new technology has been developed that uses a droplet-based microfluidic system to perform digital PCR in millions of picolitre dropletsThe microfluidic device can specifically capture cancer cells and isolate genomic DNA (gDNA) for further amplification and sequence analysis. Compared with the known wild-type gene, the genetic mutation can be detected. Using this system, the TP53 gene mutations have been detected in cervical and ovarian cancer cells. This approach can monitor multiple genetic mutations in the same small population of cells and very fell cells are required to the patients.

In recent years, the technology of melting curve analysis, as a convenient means of analyzing genetic variations, has been emerged as a potential alternative to mass spectrometry or sequencing for genotyping applications. And there is also an integrated microfluidic melting curve analysis platform for performing the single-stream genotyping assay. This platform can identify genetic mutations from crude biological samples such as whole blood. In this system, silica-coated magnetic particles are used as a solid phase for DNA extraction and transport, while specific microchip surfaces enable efficient splitting and confinement of reagents. Combined with a suitable normalization algorithm and instrumentation capable of the thermal ramp, the genetic polymorphisms can be identified in PCR amplicons.

As a global company with extensive scientific expertise, Creative Biolabs guides clients from design to product and provides continuity for their entire program. If you are interested in our services, please do not hesitate to contact us for more detailed information.

References

1. Pekin, D.; et al. Quantitative and sensitive detection of rare mutations using droplet-based microfluidics. Lab on a Chip. 2011, 11(13): 2156-2166.
2. Wang, H.; et al. A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms. Scientific reports. 2017, 7(1): 1-11.

For Research Use Only. Not For Clinical Use.