With our well-trained technical team and cutting-edge microfluidic platform, Creative Biolabs offers a range of cell culture and organ-on-a-chip models to promote your research with reliable quality and extended functionality.
MFMM1-Flow
MFMM1-Flow is an easy-to-use device for long-term 2D/3D cell culture and mechanical shear stress studies by means of microfluidics. MFMM1-Flow allows to culture cells in two separate channels and conducts 2D/3D culture experiments to study the effects of mechanical shear stress.
Features
Real-time control of shear stress and trans-wall pressure.
Compatible with any microfluidic control system.
Allows customization of channel width, height, and base material (biocompatible adhesive/plastic).
Made from oleophobic thermoplastic material and allows immunohistochemical and fluorescent detection.
Accurately simulate the mechanical environment of human vascular endothelial cells.
Provide excellent in vitro models and experimental support for vascular disease research.
MFMM1-Gradient Standard enables electrochemical gradients for 3D cell culture via three small microchannels connected between a central chamber and lateral channels.
Features
High compatibility, compatible with almost all microfluidic control systems.
Easy to use, allows immunohistochemical assays using fluorescent detection.
Cell recovery, cell cultures can be easily recovered for subsequent experiments.
Gradient controllable, enabling stable and controllable solvent concentration gradients.
Cell Culture Models
Example Application (Chemotactic Migration Studies)
MFMM1-Transflow is our versatile cell culture platform, which can be used to construct a cell-cell interface or Air-Liquid interface (ALI) to study more complex culture systems. MFMM1-Transflow uses a layer of porous membrane to separate the culture well and the microfluidic channel to form two relatively independent structures. This design can be used for the construction of ALI interface for organ chips such as lung, skin, intestine, and cornea. MFMM1-Transflow can also form a perfect endothelial-epithelial barrier for the kidney, liver, heart, and blood-brain barrier by adding medium to the culture well.
Features
Easy to use, allows immunohistochemical assays using fluorescent detection.
Cell recovery, cell cultures can be easily recovered for subsequent experiments as well as extracting the membrane with cells for histological processing.
Can be used to construct stable and simple ALI interfaces or cell-cell interfaces.
Allows customization of channel width, height, and base material (biocompatible adhesive/plastic).
Cell Culture
Example Applications (Bone on Chip and Skin on Chip)
MFMM1-DoubleFlow is an advanced device consisting of two perfusion channels connected by a porous membrane, which is your best choice for studying circulating particles, cell interactions, and organ-on-a-chip system construction.
Features
Stable cell interfaces can be constructed, enabling the construction of an organ-on-a-chip system (kidney, liver, heart, lung, blood-brain barrier, etc.).
Provide a perfect environment for the study of circulating particles (bacteria, immune responses, circulating tumor cells, etc.).
Two microchannels separated by a porous membrane.
Two different types of cells can be cultured in relatively independent environments to observe their interactions.
Allows customization of channel width, height, pore size, and base material (biocompatible adhesive/plastic).
The findings discussed in the articles related to microfluidic cell culture and organ-on-chip are presented.
1. Osteochondral Organ-On-a-Chip for Osteoarthritis Study
Fig. 1 Image of osteochondral tissues cultured on Organ-On-a-Chip.1
The study by González-Guede et al. applied organ chips to the study of osteoarthritis.1 The microfluidic chip is engineered to replicate the microenvironment found in cartilage and bone tissues, and it can sustain communication between these tissues while preserving cell viability for extended periods. The osteochondral tissue obtained from osteoarthritis patients was cultured in the organ chip system. Cartilage is a tissue that lacks blood vessels and depends on the subchondral bone for its supply of nutrients and oxygen. In the chip model discussed in the article, the subchondral bone is situated on a membrane that delivers nutrients to the cartilage. The sealed chip material guarantees that oxygen moves from the culture medium through the bone to reach the cartilage. The osteochondral tissue chip system can mimic the conditions found in joint microenvironments, with cartilage existing in a state of low oxygen and bone in a state of normal oxygen levels.
Fig. 2 Experimental configuration of osteochondral tissue-on-a-chip.1
Reference
González-Guede, Irene, Daniel Garriguez-Perez, and Benjamin Fernandez-Gutierrez. "Osteochondral Tissue-On-a-Chip: A Novel Model for Osteoarthritis Research." International Journal of Molecular Sciences 25.18 (2024): 9834. Distributed under Open Access license CC BY 4.0, without modification.
Q&As
Q: What types of cells can be used in Cell Culture and Organ-On-A-Chip models?
A: Various cell types can be used, including primary cells, stem cells, and immortalized cell lines. Cells from different tissues or organs can be cultured to create specific models tailored to the research question, enabling detailed studies of cellular and organ-specific responses.
Q: How scalable are Cell Culture and Organ-On-A-Chip models for large-scale studies?
A: Both Cell Culture and Organ-On-A-Chip models are increasingly scalable due to advancements in automation and high-throughput technologies. This scalability makes it feasible to conduct large-scale drug screening and research projects, enhancing the efficiency and impact of biomedical research.
Q: What quality control measures are in place for Cell Culture and Organ-On-A-Chip experiments?
A: Quality control measures include validating cell viability and functionality, ensuring proper device fabrication and fluid dynamics, and regularly monitoring cellular responses. These measures are crucial to ensuring the reliability and reproducibility of experimental data.
Q: Can these models simulate the immune response?
A: Yes, both Cell Culture and Organ-On-A-Chip models can incorporate immune cells to study their interactions with other cell types and tissues. This is particularly useful for research on immune responses, autoimmune diseases, cancer immunotherapy, and infectious diseases.
Q: How do these services integrate with other omics technologies?
A: These models can be combined with genomics, proteomics, and metabolomics to provide a multi-dimensional analysis of cellular and organ responses. This integration allows for a comprehensive understanding of biological processes and the identification of biomarkers, enhancing the depth and breadth of biomedical research.
Resources
Videos
Creative Biolabs has established a comprehensive microfluidics technique platform.
Fig. 1 Image of osteochondral tissues cultured on Organ-On-a-Chip.1
Fig. 2 Experimental configuration of osteochondral tissue-on-a-chip.1
