Creative Biolabs is a world-leading services provider in the field of microfluidic chip development. We are dedicated to supporting the synthesis of alginate particles by designing and developing a high-quality and high-sensitivity microfluidic chip system for our customers all over the world.
Alginate is a natural, biocompatible, and highly stable carbohydrate-based hydrogel particle. In general, alginate is a relatively suitable type of material used as a drug carrier and encapsulation material and as fabricate scaffolds for regeneration medicine because of its high biocompatibility, biodegradability, and availability. It can be polymerized by simple gelation with divalent cations such as calcium ions. However, conventional alginate particles have limitations such as low encapsulation efficiency because of drug leaching during bead preparation and burst release in high pH values. Therefore, the synthesis of alginate particles with multiple composites is essential to meet the various requirements of different biological applications.
Microfluidics has emerged as a versatile method for generating micrometer-sized particles with controllable size and functionality in recent years. The use of microfluidic chips to construct more complex structures may resolve the problems of the traditional synthesis of alginate particles. An on-chip approach can be used to synthesize alginate particles with adjustable pore sizes to achieve a fine-tunable release profile of the encapsulated bioactive agents.
In particular, changing the flow rate could easily control the diameter of the hollow alginate fibers and the synthesis of cell-laden and protein-immobilized alginate hollow fibers and in situ alignment of fibers. For example, flat-shaped alginate microfibers with grooved microstructures can be synthesized to build scaffolds for cell alignment. The results showed that the cells cultured on groove fibers have rather excellent behaviors of alignment.
Fig.1 Flat-shaped alginate microfiber prepared by the microfluidic method. (Wang, 2018)
Numerous therapeutic materials can be quickly loaded during the fabrication process, and the resulting fibers have great potential for use in the delivery of drugs and other therapeutic materials. As an initial application of these hollow fibers, vascularized tissues can be emulated by embedding endothelial-cell-laden hollow fibers into smooth muscle cell-laden AGF hydrogels. This microfabrication process is a promising method for engineering vascular tissue and studying cell-cell interactions in 3D structures.
Fig.2 Schematic of coculturing of alginate hollow fiber to emulate vascularized tissue. (Lee, 2009)
Creative Biolabs offers custom services in microfluidic development by microfluidic technology coupled to our scientists' equally extensive expert knowledge. We have served various industries offering comprehensive microfluidic research and development services based on specific client needs. If you are interested in learning more about our solutions, please do not hesitate to contact us.
For Research Use Only. Not For Clinical Use.