• Home
• SERVICES
• One-Stop Microfluidic Solutions
• Droplet Generator and Flow Chemistry
• Particle Synthesis
• Lipid and Liposome Particle Synthesis

Creative Biolabs is a world-leading services provider in the field of microfluidic chip development. We are dedicated to supporting the synthesis of lipid and liposome particles by designing and developing a high-quality and high-sensitivity microfluidic chip system for our customers worldwide.

Synthesis of Lipid and Liposome Particles

Lipid and liposomes are vesicular structures consisting of one or more lipid bilayer membranes that encapsulate an aqueous volume. Due to the unique properties, lipid and liposomes are widely used as drug and gene delivery vehicles. Several traditional methods have been developed to prepare lipid and liposomes, such as thin-film hydration, detergent dialysis, reverse-phase evaporation, and ethanol injection. However, limitations of these conventional methods include complexity and length of procedures, low drug encapsulation efficiency, polydisperse size distributions.

Fig.1 Drug loading in liposome particles.¹

Microfluidics Development for Synthesis of Lipid and Liposome Particles

Microfluidics is a novel technology for the production of micro-and nanosized lipid and liposomes. The self-assembly in microfluidics is controlled by varying liquid flow rates, ratios of cross-flows, and lipids' composition and concentration, resulting in tunable sizes and narrower size distributions. Therefore, microfluidics provides a new platform for developing and optimizing lipid and liposomes in the emerging field of nanomedicine.

• Example 1

A microfluidics injection device driven by a syringe pump can be used to produce liposomes under various conditions. This microfluidic method is similar to the conventional ethanol injection method. Still, it enables continuous manipulation of particle size by providing well-defined mixing and a constant water-to-ethanol flow ratio. This low-cost and straightforward method can be facilely used in the laboratory with the critical features of microfluidics but without the need for microfabrication.

• Example 2

Researchers have reported on the controlled formation of sub-micrometer-sized liposomes in a microfluidic channel network via hydrodynamic focusing. The liposomes are formed by a diffusively driven process when a stream of lipids dissolved in an organic solvent is hydrodynamically sheathed between two oblique buffer streams in a microfluidic channel. In this initial study, the diameters of liposomes produced using this method can be controlled from 50 nm to 150 nm.

Available Methods for Synthesis of Lipid and Liposome

Fig.2 Microfluidic Liposome Particle development.²

Advantages of Microfluidic-based Synthesis

The characteristics of lipid and liposome particles formulations produced by microfluidic systems have distinct advantages over traditional methods, such as:

• Reproducible control of the size and size distribution
• Quickly form nanosized liposomes with smaller size and narrower size distribution
• Uniform particle size and good colloidal stability
• Simple, quick, and affordable, and easy to use in the laboratory
• No need for the costly microfabrication equipment and advanced manipulation skills
• Minimize sample consumption without adversely affecting the encapsulation efficiency

Our Microfluidic Services

Over the past decade, microfluidic technologies have been developed as necessary chemical synthesis and biological analysis tools. In parallel, there has been an increasing interest in using microfluidics as a novel platform to prepare nano- and microparticles such as lipid and liposome particles. Creative Biolabs is a global provider with the objective to use microfluidic technologies to synthesize lipid and liposome particles in order to meet the growing needs for the development of microfluidic chips. Our services can be tailored to our client's specific needs. Please contact us for more detailed information; we look forward to discussing your inquiry and finding the best solution for your needs.

References

1. Nakhaei, Margiana, et al. " Liposomes: structure, biomedical applications, and stability parameters with emphasis on cholesterol." Frontiers in Bioengineering and Biotechnology 9 (2017): 705886.
2. Bostanabad, Puliga, et al. " Microfluidic Manufacture of Lipid-Based Nanomedicines." Pharmaceutics 14.9 (2022): 3390.

For Research Use Only. Not For Clinical Use.