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The microfluidic technology developed in the early 1990s initially studied and manipulated liquid transport rules on the micrometer and nanometer scales. With the continuous development of this technology, microfluidic technology is widely used in life science and medical research and has achieved remarkable results. Creative Biolabs combines its rich biomedical research experience with microfluidic technology to provide customers with microfluidic chip design and customization services for specific needs.

Microfluidic Chips for Small Molecule Analysis

Microfluidic chips have gradually become powerful tools for biological analysis in the fields of fundamental biology, clinical applications, food safety, and environmental monitoring due to their high sensitivity, high throughput, and low cost. Among them, the timely detection and analysis of biologically active small molecules such as oligonucleotides, oligopeptides, oligosaccharides, vitamins, minerals and metabolites in physiological processes are very important for guiding therapeutic medication and predicting disease prognosis. Small biomolecules are usually present in the body's tissues, organs, bones and tears, saliva, sweat, urine and blood. And because the sampling of body fluids can minimize intrusion into the human body, body fluids become ideal sampling specimens. Different biological fluids have different components and may have different effects on the test. Therefore, it is necessary to design corresponding microfluidic chips according to the specific conditions of various biological fluids to achieve high accuracy detection and analysis.

Fig. 1 The advantages of microfluidic chip for cancer biomarker detection.^1,2

Microfluidic Chips for Small Molecule Analysis in Other Biological Fluids

Blood and sweat are two common and important body fluids. These two samples are often used for the detection of glucose and other small molecules. In addition, urine, saliva, tissue fluid, nasal discharge, semen, gastric juice, vaginal secretions, and saliva can also be used as samples for health monitoring. Similar to the basic principles of other microfluidic chips, microfluidic chips used to detect small molecules in biological fluids are also based on micro electromechanical system (MEMS) technology. A large laboratory system is scaled down on a glass or plastic substrate to replicate the entire process of biological and chemical reactions and enable fast and automatic detection. Its distinguishing feature is that it can construct channels, reaction chambers and other functional components on the micron scale to accommodate fluids, and can operate the movement process of micron volume fluids in tiny spaces.

Solutions from Creative Biolabs

Taking the detection of cortisol in saliva as an example, Creative Biolabs has developed a microfluidic detection technology based on aptamer functionalized gold nanoparticles, which does not require target labeling or a capture probe to be fixed on the detection surface, and omits washing steps. The recognition system of this technology is aptamer functionalized gold nanoparticles pre-integrated with electro-active triamcinolone. Cortisol can be competitively combined with aptamer, and by using square wave voltammetry in the microfluidic chip to track the signal of the replaced triamcinolone, the cortisol level can be detected. Validation tests show that the cortisol concentration measured by this method is quite consistent with the ELISA results, but it greatly reduces the test time and the numbers of samples.

Creative Biolabs’ Strengths in Development of Microfluidic Chips

• Years of research experience in microfluidic technology
• The top-class expert from engineering, physics, chemistry, micromachining and bioengineering
• Extensive accumulation and experience in the biomedical field
• Proprietary and stable microfluidic chip manufacturing technology
• Competitive delivery time and price
• Perfect guidance and after-sales service

With reliable research and development background, Creative Biolabs can provide customs with high-quality design and customization services for the development of microfluidic chips, especially for the detection of small molecules in less common biological fluids. If additional help is needed, please directly contact us and consult our technical supports for more details.

References

1. Guo, Zhang, et al. " Multifunctional microfluidic chip for cancer diagnosis and treatment." Nanotheranostics 5.1 (2021): 73-89.
2. under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.