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Microfluidics offers technology to enable the miniaturization of biological assays, significantly improving the analysis efficiency of samples. The micro-channels are generally small in cross-sectional area and can run many samples in parallel. This parallelization, in conjunction with the small volumes required for a given test, facilitates high-throughput analysis. Creative Biolabs is dedicated to establishing the most exquisite microfluidic platform for our clients and the one-stop microfluidic solutions can provide comprehensive technical support for advancing our clients’ projects.

Why Biomarkers Detection is Important?

Blood harbors a massive amount of information about the functioning of all tissues and organs in the body. Every minute, the entire blood volume is recirculated throughout the body, delivering oxygen and nutrients to every cell and transporting products from and toward all different tissues. As a result, blood sampling and analysis are of prime interest for both medical and science applications, and hold a central role in the diagnosis of many physiologic and pathologic conditions, localized or systemic.

Microfluidic chips are extremely attractive for blood analysis. For drug discovery, microfluidic chips may redefine the entrance criteria for clinical trials and test for these criteria in a time- and cost-effective way. In small-animal studies, microfluidic chips would only use minute amounts of blood for analysis, allowing for repetitive sampling at multiple time points and minimizing the adverse effects of blood drawing. On the whole, on-chip blood sample preparation and analysis would lead to more gentle, fast, and consistent manipulation of the living cells, and therefore more accurate and better quality of extracted information.

Fig. 1 Blood cell sorting and analysis.^1,3

Services at Creative Biolabs

Creative Biolabs offers comprehensive microfluidic chips development services for blood sample preparation and analysis.

• Microfluidic chips for blood sample preparation

There are two distinct approaches to the preparation of blood samples based on the number of cells simultaneously processed. In the serial approach, cells are aligned in a single row, identified as they pass one by one in front of a detector module, and immediately separated using a distinct module. In the parallel approach, cells are first positioned in a regular array and then simultaneously identified and separated in pure subpopulations.

• Microfluidic chips for blood sample analysis

Various microfluidic devices for a wide range of assays in small volumes, incorporating reaction chambers, separation columns, micropumps, and valves, as well as other components, have been developed. Proteins can be directly captured, isolated, and identified on chip using techniques such as electrophoresis, isoelectric focusing, and mass spectrometry. Microfluidic chips already exist for a range of nucleic acid analysis, including, but not limited to, PCR, gel electrophoresis, and hybridization assays.

Creative Biolabs has focused on the development of microfluidic chips for years, we whole-heartedly cooperate with you to accomplish our shared goals. Our team provides you with outstanding support and meets your specific needs with a professional technology platform. If you are interested in our services, please contact us for more details.

Published Data

The following are results highlighted in articles related to blood analysis conducted on a microfluidic chip:

1. Nano-Interstice Guided Extraction of Blood Plasma Without Power in a Microfluidic Device with an Integrated Membrane Filter.

Fig 2. Overall illustration of the plasma separation microfluidic device.^2,3

Extracting plasma from whole blood is a prerequisite for detecting biomarkers in blood. Jaehoon Kim et al. proposed a simple and fast microfluidic device with integrated membrane filter.^2,3 The nanogaps located on either side of the channel of the chip can efficiently draw the extracted plasma from the membrane. After verification, the plasma extraction rate of this device is very high, about 45%, and it successfully supplies 20 μL of extracted plasma within 16 minutes. Compared with the conventional plasma extraction method in the past, the extraction efficiency can be increased by 20-25%, and no external instrument is required.

References

1. Jiang, Zhao, et al. " An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells." Scientific Reports 7 (2015): 42612.Park, Son, et al. " High-Throughput Microfluidic 3D Cytotoxicity Assay for Cancer Immunotherapy (CACI-IMPACT Platform)." Frontiers in Immunology 10 (2019): 1133.
2. Kim, Jaehoon, et al. "Nano-interstice driven powerless blood plasma extraction in a membrane filter integrated microfluidic device." Sensors 21.4 (2021): 1366.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.