Microfluidic Development Service for Protein Rapid Enzymolysis Analysis

Inquiry

Creative Biolabs offers a suite of custom microfluidic chip development services to address the ever-changing research of proteomics. Based on our extensive experience and advanced platforms, now we combine protein rapid enzymolysis analysis technology for better proteomics research.

Introduction of Proteome Research

Proteomics is the large-scale study of the entire set of proteins that are produced or modified by an organism or system. The technology of proteome research has been widely used in different fields, especially for the clinical diagnosis of human major diseases. Compared with gene research, the technology of protein research is complicated and difficult due to the multiple amino acid kinds and complicated posttranslational modification. There are three kinds of technology paths in numerous proteomics research schemes, which are the Top-Down technology path, Short-Gun technology, and the technical route combining the above two methods. Among them, proteolysis is a necessary step for protein identification. However, the traditional method uses the off-line mode that the albumen after separating is carried out enzymolysis mostly, which results in contamination and loss of protein samples and also greatly reduces work efficiency.

Fig. 1 Microfluidic enzyme kinetics analysis.¹

Microfluidic Chips Development for Protein Rapid Enzymolysis Analysis

A liquid drop microsystem has been developed for protein analysis, which is combined with multiple protein isolation technics and electrospray ionization mass spectrum. This system enables rapid separation of proteins and high-throughput enzymatic hydrolysis, as well as avoids cross-contamination, so as to realize the automation and high-throughput of proteomics research. Using the microsystem liquid drop generation technology, the mixture of the separated protein sample and the protease solution can form water-in-oil droplets in the microchannel with the oil phase. In summary, this system contains a protein separation device, drop microreactor, electron spray shower nozzle, temperature control equipment, and the identification of fusion protein device. Among them, the described drop microreactor can be a kind of microfluidic chip, which is always composed of micro reaction channel, oil phase inlet, protein solution import, protein enzyme solution import, pH value adjusting mouth, and electron spray shower nozzle connector. Based on a series of advantages, the reactor provides more detailed and comprehensive protein information for proteomics research.

As forward-looking research as well as a leading custom service provider in the field of microfluidic chip development, our pipeline is built to meet the customer’s project and assay-specific requirements for our customers all over the world. If you are interested in our services, please do not hesitate to contact us for more detailed information.

Reference

Neun, Vliet, et al. " High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform." Anal. Chem. 94.48 (2022): 16701-16710.

For Research Use Only. Not For Clinical Use.