Microfluidic chip technologies have been widely applied in the analysis of amino acid due to its advantages such as low sample consumption, short analysis time, high throughput and compatible size with most biological samples. Creative Biolabs is a world’s leading service provider who is devoted to offering all-around and low-cost microfluidic chip development services to accelerate amino acid analysis by employing our expertise and advanced technology platforms.
Amino acid analysis is an essential part of analytical biochemistry and important for the identification and quantification of proteins or peptides. However, there have several challenges in amino acid analysis: firstly, amino acids are difficult to detect in their natural form due to lacking a chromophore or electroactive group; secondly, it is required to separate D/L isomers because they are optically active; thirdly, a large amount of interferences in sampling material posses a major challenge for sample preparation/separation/detection. Although a number of articles have reported amino acid analysis by liquid chromatography, optical detection, capillary electrophoresis (CE), there have some limits to these methods. The emergence of microfluidic technologies has attracted a lot of attention for amino acid analysis due to many advantages. Unlike some traditional methods, the microfluidic chip technology integrates the whole analytical process including preconcentration, derivatization, separation, and detection on one platform, greatly reducing sample and reagent consumption, improving detection capability, and increasing detection throughput.
Fig.1 Microfluidic Chip.
Microfluidic devices have been widely applied for derivatization, separation, and detection of amino acids. Microfluidic device with precolumn or postcolumn derivatization chambers could improve the detection sensitivity of amino acids with electrochemical or optical methods. For separation, there are several separation modes have been developed on microfluidic devices for separation of amino acids such as microchip zone electrophoresis (μCZE), microchip electrochromatography (μCEC), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC). For example, a PDMS-based μCE system has been been used to separate amino acids in aqueous solutions with resolutions comparable to those obtained using fused silica capillaries. A microfluidic device coupling with γ-CD-MEKC separation model was employed to separate FITC-labeled amino acids with efficiencies up to 28 000 plates. Besides, a PDMS microfluidic device combining with a gel monolithic column was also applied for rapid separation of FITC-labeled amino acids. Importantly, microfluidic devices also can be used to detect amino acids via in combination with some sophisticated miniaturized detection systems such as carbon nanotube (CNT) film modified electrodes, pulsed amperometric detection (PAD), and MS. Scientists employed a copper electrode on a microfluidic device for the detection of amino acids with limits of detection at the micromolar level.
Fig.2 Image of a microchip with a spiral separation channel. (Feng, 2009)
With years of experience in the field of microfluidic chip development services, Creative Biolabs is confident in offering a series of high-quality and high-performance microfluidic chip development services to promote your success. Our excellent scientists work closely with you and custom optimal microfluidic chip development solutions to meet your project’s special requirements. We not only support the design, development, and production of high-quality microfluidic chips fabricated with various kinds of derivatization, separation, and detection technologies but provide the development of microfluidic chip-based assay services for analysis of various biomolecules including proteins, nucleic acid, enzymes and their inhibitors, amino acid as well as small molecules. If you are interested in our services, please contact us to discuss your project.
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