Microfluidic technology has many advantages over other conventional immunoassays such as high throughput, short analysis time, small volume, and high sensitivity. In particular, the combination of microfluidic technology and multiplex microbead-based immunoassay greatly improves sensitivity and efficiency of detection. As a leader of microfluidic technology fields, Creative Biolabs is proud of offering a full range of microfluidic based immunoassay and microfluidic chip development services to support multiplex analysis of DNA, RNA, antibodies, antigens, and a vast number of other biological molecules.
Multiplex microbead-based immunoassays possess many advantages over some conventional immunoassay methods such as western blot and ELISA. They allow simultaneous detection of multiple biomolecules in one reaction vessel, greatly improving detection efficiency, shortening analysis time, decreasing sample and reagent consumption. The combination of microfluidic systems with microbead-based immunoassays provides a powerful platform for multi-analyte analysis. Compared with normal fluidic systems, the usage of microbeads greatly increases the surface to volume ratio in a microfluidic device and improves the sensitivity of assays by increasing interactions between samples and reagents. Moreover, analytes attached to the beads can be easily transported in a fluidic system with pressure-driven flow or electric fields. Although flow cytometry is a popular alternative to immunoassays with its advantages of short analysis time, low sample size, high sensitivity, and multiplexing ability with the use of encoded microbeads as solid supports, it is expensive, bulky, and cannot be integrated into a total analysis system like a fluidic chip. While microfluidic technologies can integrate all preparation, detection and analytical processes into a single mini-sized system, greatly simplifying detection and analysis procedure.
Table.1 Comparison of immunoassays.
|Bead-based microfluidic immunoassays||Flow cytometry||ELISA|
|Procedure||Integrating all preparation, detection and analytical processes into a single mini-sized system||Requiring additional sample preparation steps||Numerous washing and preparation steps, long diffusion time required for antigen-antibody binding|
Bead-based microfluidic immunoassays have attracted a large amount of interest in various biological assay applications.
Fig.1 Schematic illustration of microfluidic bead-based immunoassay (µFBI). (Yu, 2010)
Creative Biolabs is dedicated to supporting multiplex immunoassay services based on bead-based microfluidic technologies. Our experienced scientists can design and develop different types of bead-based microfluidic immunoassays to support a variety of biomolecule detection such as proteins, DNA, small molecules. If you are interested in our services, please contact us to discuss your project.
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