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Multiple emulsions (MEs) contain a drop laden with many micro-droplets, which can encapsulate various agents, such as cells, proteins, nucleic acids and hydrophilic or lipophilic molecules to produce microtissue structures, control chemical reactions, deliver chemicals or drugs, and regulate cell fates. At Creative Biolabs, microfluidics has been developed as one alternative and versatile tool to generate uniform and size-controlled droplets and emulsions. Microfluidic-based synthesis process of MEs has been presented by us to provide a rapid and controlled generation of monodisperse MEs.

Microfluidics for MEs Production

Microfluidics has been widely applied in a wide variety of fields, such as material synthesis, disease diagnostics, biosensors, protein crystallization, gene expression, drug and gene delivery, single-cell analysis, etc. In the emulsion formation field, microfluidics has attracted intensive interest with the capacity of generating monodisperse single, double and multiple emulsions with uniform size, narrow size distribution and desired properties. The size and number of the encapsulated emulsions can be precisely manipulated by controlling the flow rate of the dispersed and continuous phases. At present, the most important microfluidic configurations for droplet generation and emulsion formation are T-junction, co-flowing junction, and flow-focusing junction.

Fig. 1 Droplets microfluidic platforms for generation of emulsions (A) T-junction; (B) co-flowing junction; and (C) flow-focusing junction. ^1,3

Advantages of Microfluidic Emulsification Strategy

Compared with conventional emulsification methods, microfluidic emulsification strategy offers the fabrication of MEs with complex drop morphologies and low polydispersity (less than 5%) in a highly reproducible manner. Microfluidic emulsification has several major advantages:

• The size of the emulsions is at each hierarchical level with narrower particle size distribution.
• The number and the type of inner drops nested inside each outer drop are highly consistent.
• The internal structure and shape of outer drops are highly consistent.
• The rate of droplet generation is precisely controlled.
• Higher reproducibility with a low batch-to-batch variation.
• The encapsulation efficiency is high up to 100% with accurate and independent control.

Applications of MEs

MEs have been novel carrier systems which are complex, polydisperse, multiphase systems consisting of at least two immiscible liquids simultaneously in a single system. They are promising tools applied in the following areas:

Featured Services at Creative Biolabs

With years of experience and advanced microfluidic platforms, Creative Biolabs is continuously optimizing our microfluidic devices for the optimization of MEs production with high yields, low polydispersity and well-defined diameters. Our versatile microchip-based synthesis process of MEs is presented to provide a rapid and controlled generation of monodisperse MEs. We are pleased to offer the best-qualified microfluidic chips with the capability of highly controlled and high-throughput MEs formation.

Fig. 2 Microfluidic production of multiple emulsions. ^2,3

If you are interested in our services, please do not hesitate to contact us for more detailed information.

References

1. Damiati, S.; et al. Microfluidic devices for drug delivery systems and drug screening. Genes. 2018, 9(2): 103.
2. Vladisavljević, G. T.; et al. Microfluidic production of multiple emulsions. Micromachines. 2017, 8(3): 75.
3. under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.