Exosomes are the smallest extracellular vesicles (approximately 30–150 nm) and are generally packaged with lipids, proteins, functional messenger RNA and microRNA, and double-stranded DNA from the cell which they originated, as a key participant in intercellular communication. Because of its ability to protect information molecules from degradation in body fluids, it has become an attractive candidate for innovative diagnostic and therapeutic applications. However, routine isolation and analysis of high-purity exosomes in clinical settings is challenging. Traditional methods face many disadvantages, including low yield and/or purity, long processing time, high cost, and difficulty in standardization.
To solve these problems, Creative Biolabs provides a solution with great development potential through our microfluidic technology platform, combining many separations and sensing functions for exosome separation, detection and analysis, focusing on Medical and clinical applications. Our services not only promote basic research, but also pave the way for routine exosome-based liquid biopsies in personalized medicine.
Fig.1 Exosomes represent a subset of extracellular vesicles with characteristic size in the ~30–150 nm range. (Contreras-Naranjo, 2017)
Due to the important role of exosomes in coordinating cell-to-cell communication and molecular exchange, it has become one of the focal areas in biological and medical research. Exosomes contain personalized information related to disease status, treatment response, exposure to environmental cues, and countless other health factors, which are present in vesicle packaging that can be collected from body fluid samples. But the biggest challenge at present is the lack of effective and standard techniques to isolate and analyze clinical-grade exosomes, difficulties that are compounded when dealing with raw biological fluids that inherently contain a high proportion of proteins, other extracellular vesicles, and cells with similar physical and/or biomolecular characteristics as exosomes. Current isolation methods either rely on the difference in size between exosomes or on specific surface markers. Conventional techniques based on these principles include ultracentrifugation, precipitation, filtration, chromatography, and immunoaffinity-based methods.
Based on comprehensive Microfluidic technology platforms, Creative Biolabs provides one-stop Microfluidic chip development services for exosome isolation.
If you want to inquire more about our microfluidics-based exosome isolation services, please contact us.
For Research Use Only. Not For Clinical Use.