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Microbiomes exert a significant influence on human health and diseases; they have been shown to be linked to dysregulation of the gut microbiota, including neurodegenerative, cardiovascular, and metabolic diseases. Microfluidic technology has been used to study ecological and evolutionary processes in microbial communities by encapsulating microbial communities into microdroplets. Creative Biolabs has extensive experience in offering custom microfluidics development services to advance the precise encapsulation of microbiomes. We would try our best to provide a customized proposal to meet your project requirements in the quality, timeline, and budget manner.

Microfluidics for Microbiome Research

Microfluidics has made a significant impact in single-cell analyses, molecular diagnostics, and in vitro tissue models. Similarly, microfluidics can value add to different aspects of microbiome research. Continuous microfluidics has been widely used in microbial single-cell culture, whole-genome sequencing (WGS), gene expression, and metabolic analyses. Droplet-microfluidics, in which droplets of sub-nanoliter volumes are generated and used as individual chemical reactors, is becoming a new trend for its high-throughput single microbial cell or microbiome encapsulation ability.

Table.1 Summary of reported microfluidic-based host-microbe models developed for different applications. (Tan, 2020)

Microfluidics Enabled Highly Parallel Co-Cultivation of Microbial Communities

Park, et al. developed a microfluidic device for encapsulating and co-cultivating subsets of a microbial community in monodispersed droplets, in which encapsulated microbes can grow only if the droplet localizes symbiotic interactions. This device is consisted of a slanted T-junction for droplet generation (generating monodispersed droplets with a single vacuum line at the outlet) and a chamber to hold droplets for cultivation. The achievable range of frequency was 1-500 droplets/sec. This device can be used to discover synergistic interactions among microbes and mimic various compositions of natural microbial communities.

Fig.1 A microfluidic device for microbial co-cultivation. (Park, 2011)

Microfluidics Development Services

As a pioneer and the undisputed global leader in the field of microfluidic development, Creative Biolabs offers high-quality microfluidics development services to advance the precise encapsulation of single cells, proteins, bacteria, viruses and other compounds. Our flexible microfluidics development services for microbiome encapsulation precisely depend on your specific project requirement.

• Integrated microfluidics devices

Used for (i) isolation, purification, and encapsulation of microbiomes from clinical specimens such as stool and saliva; (ii) sample preparation for NGS sequencing with high sample quantity and throughput during metagenomic analysis, benefiting the identification of microbiome components and metagenome wide association studies (MWAS).

• Multiplexable microfluidics devices

Used for culture and functional measurements of various microbes in the microbiota to facilitate mechanistic understanding of causal relationships. Microfluidic droplets can co-encapsulate microbes with different functional reporters.

Microfluidics has rapidly emerged as one of the key technologies opening up new experimental possibilities in microbiology. Microfluidics-based microbiome encapsulation can precisely encapsulate microbiomes within monodisperse microdroplets for the study of microbiome-host interactions and therapeutic mechanisms. Creative Biolabs has developed various microfluidics systems to benefit cell, protein and microorganism encapsulation. If you are interested in our services, please do not hesitate to contact us for more detailed information.

References

1. Tan, H. Y.; Toh, Y. C. What can microfluidics do for human microbiome research? Biomicrofluidics. 2020, 14(5): 051303.
2. Park, J.; et al. Microdroplet-enabled highly parallel co-cultivation of microbial communities. PloS one. 2011, 6(2): e17019.

For Research Use Only. Not For Clinical Use.