• Home
• SERVICES
• One-Stop Microfluidic Solutions
• Droplet Generator and Flow Chemistry
• Encapsulation Services
• Enzyme Encapsulation

Creative Biolabs is an outstanding CRO that integrates its solid knowledge in microfluidics and powerful technology platform to support a variety of microfluidic-based enzyme encapsulation services. We help you design and development an optimal microfluidic based enzyme encapsulation solution tailored to your special requirements. We also support one-stop microfluidic-based enzyme encapsulation production, detection, and analysis services to accelerate your success.

Advantages of Enzyme Encapsulation

Encapsulation is one of the most promising strategies for enzyme immobilization. Enzyme encapsulation is to entrap inside a small membrane vector that allows the substrates and other small molecules to pass through but retains the enzyme. There have several advantages for enzyme encapsulation, including:

• Stabilize native enzyme structures and activity
• Protect enzymes from degradation
• High enzyme-substrate ratio
• Minimize reaction time
• Reduced opportunities for contamination in the enzymatic reaction
• Simplify the process of enzyme recovery and greatly decrease the cost of enzyme production

Microfluidic-based Enzyme Encapsulation

Although enzyme encapsulation is a simple, gentle, and general method for immobilizing enzyme, it may exist several limitations such as low enzyme loading efficiency, slow diffusion of the substrate to the enzyme, enzyme leakage, and enzyme contamination by encapsulation materials. The emergence of microfluidic techniques provides a new attractive landscape for encapsulating enzymes in view of many advantages.

• High encapsulation efficiency: the microfluidic technique integrated on-chip encapsulation, gelation, and de-emulsification into a one-step fabrication process that enables scalable enzyme encapsulation while retaining the functionality and activity of loaded enzymes.
• Production of controllable enzyme-loaded nanoparticles: microfluidic technologies allow the production of the nanoparticles with versatile and controllable structures for enzyme encapsulation.
• Good repro­ducibility and consistency: microfluidic approaches enable the continuous reproducible production of the encapsulated enzyme in uniformly-sized particles.
• Cost-efficiency: decreasing the consumption of enzymes, substrate, and encapsulation materials.
• High-quality encapsulation: protecting enzymes from degradation, decreasing enzyme leakage, and reducing opportunities for contamination in the encapsulation process.

Application of Microfluidic-based Enzyme Encapsulation

Microfluidic technologies have a wide range of applications in enzyme encapsulation. Microfluidic-based enzyme encapsulation reactors provide a useful and powerful tool for performing biocatalytic reactions, with many advantages over traditional technologies, such as low energy consumption, high efficiency, rapid heat exchange, fast mass transfer, and superior repeatability. Sakai-Kato et al. (2003) developed an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip, integrating tryptic digestion, separation, and detection. The device greatly shortened analytic time and increase operational stability. Besides, WU et al. (2004) also developed Titania and alumina sol-gel based PDMS microfluidic reactor with trypsin encapsulation, providing an effective platform for peptide mapping with shortening digestion time and increased operation stability. Interestingly, microfluidic-based enzyme encapsulation is also applied for disease treatment by stabilizing therapeutic enzyme structure and function. A recent study reported a glass-capillary microfluidic device for encapsulating therapeutic enzymes in liposomes. The encapsulated enzymes exhibited a good enzymatic activity. In vivo assays showed therapeutic enzyme-loaded liposomes present an effective anti-inflammatory property, which is promising for the treatment of local inflammation.<

Fig. 1 Microfluidic encapsulation of enzymes within lipid nanoparticles.^1,2

Services at Creative Biolabs

Creative Biolabs is an excellent and world-leading service provider that is devoted to helping our clients to design and develop top-quality microfluidic chips to promote their research. Equipped with state-of-the-art development facilities and comprehensive expertise, we support a widely full range of microfluidic-related enzyme encapsulation services including the development and production of versatile and controllable nanoparticles, enzyme encapsulation with certain homogenization characteristics, and development of microfluidic-based enzyme encapsulation reactors. We have accumulated extensive experience in several important projects of microfluidic-based development and biomolecule detection. Please contact us to let us help you design and develop the best effective microfluidic chip technologies for enzyme encapsulation.

References

1. Weaver, Sommonte, et al. " Microfluidic encapsulation of enzymes and steroids within solid lipid nanoparticles " Drug Delivery and Translational Research 14 (2024): 266-279.
2. Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.