{"id":147,"date":"2022-05-28T09:23:45","date_gmt":"2022-05-28T09:23:45","guid":{"rendered":"https:\/\/microfluidics.creative-biolabs.com\/blog\/?p=147"},"modified":"2023-09-06T02:30:40","modified_gmt":"2023-09-06T02:30:40","slug":"organ-chip-based-detection-technology-part-4","status":"publish","type":"post","link":"https:\/\/microfluidics.creative-biolabs.com\/blog\/organ-chip-based-detection-technology-part-4\/","title":{"rendered":"Organ Chip-Based Detection Technology – Part 4"},"content":{"rendered":"

As a new type of cell experiment tool, the organ chip integrates chip technology and chemical analysis technology, and forms a 3D organ model system in vitro<\/em> based on different principles such as cell self-assembly and engineering design, which can partially overcome the limitations of traditional animal models. It has gradually become an important drug evaluation model.<\/span><\/p>\n

\"\"Organ-on-a-chip Applications<\/span><\/p>\n

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As a professional agent providing organ chip research and development services, Creative Biolabs provides various service types for organ chip development, testing, and evaluation. Taking advantage of advanced technology platforms and a professional research team, the company has established an organ chip detection service system involving various fields such as medical evaluation and transport function determination.<\/span><\/p>\n

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Detection Technology Based on Organ Chips<\/strong><\/span><\/p>\n

Secretion\/effluent assay<\/strong><\/span><\/p>\n

Organ chip-based secretions\/effluents include peptides, proteins, amino acids, miRNA, DNA, RNA, etc. Compositional analysis of these microparticles allows for rational inferences about cell growth, testing response to drugs, or high-throughput drug screening, among others. In addition, the measurement of secretions can also provide potential treatment methods for diseases. For example, for some diseases that are not easy to be directly administered to target organs, the secretion of hormones in other organs can be adjusted to indirectly treat diseased organs. Measurement of secretions\/effluents by an organ chip-mass spectrometry platform allows cell secretions to be recovered during cell culture for component composition analysis. This technique enables kinetic studies over a sustained period of time to determine cell viability and function. The introduction of high-resolution mass spectrometry provides a powerful means for the qualitative and quantitative detection of signaling factors in cell signaling. It can also test the direct impact of drugs on the human microbiome before medication, which is helpful for the development of personalized medicine.<\/span><\/p>\n

\"\"\u00a0Microfluidic perfusion system for insulin secretion fingerprint analysis<\/span><\/p>\n

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Functional Transport Studies<\/strong><\/span><\/p>\n

Creative Biolabs can provide a variety of drug transport function studies to evaluate the effectiveness and safety of new drugs. Currently, a series of transport function tests for the liver, intestine, and peritoneum are provided. Take Caco-2 cell-based intestinal transit research as an example, Caco-2 cells were first cultured using chip membranes, which showed a low permeability coefficient when cyclophosphamide was absorbed by the intestinal wall. Therefore, by comparing the permeation parameters, the translocation can be accurately judged. In addition, the joint use of multiple analytical instruments can further ensure the reliability and comprehensiveness of the data, such as high-performance liquid chromatography (HPLC), real-time fluorescent quantitative nucleic acid amplification detection system (qPCR), and enzyme-linked immunosorbent adsorption (ELISA).<\/span><\/p>\n

\"\"\u00a0Mathematical Simulation of Metabolic Reactions and Transport Phenomena in Liver-on-a-Chip<\/span><\/p>\n

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Determination of differentiation status<\/strong><\/span><\/p>\n

Organ chips can simulate the morphology, proliferation, differentiation, and migration of cells in vivo<\/em>, and can conduct research on different disease model states in a similar tissue microenvironment. For example, human embryonic stem cells (ES) and induced pluripotent stem cells (iPS) can be induced to differentiate to adapt to specific needs for organ-on-a-chip development. The differentiation state of cells can be characterized by the activity of alkaline phosphatase (ALP).<\/span><\/p>\n

\"\"Differentiation of Human Neuroepithelial Cells in Organ Chip Microchannels<\/span><\/p>\n

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ALP activity detection methods include but are not limited to:<\/span><\/p>\n