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Creative Biolabs is a world-leading services provider in the field of microfluidic chip development. Equipped with advanced platforms and powerful technologies, we are dedicated to supporting bacteria encapsulation services by developing a high-quality and high-performance microfluidic chip system.

Advantages of Bacteria Encapsulation

Encapsulation has become an effective method to protect bacteria against severe environmental factors. In the field of probiotic bacteria, encapsulation emerges as a biotechnological strategy that provides several significant advantages over the use of free probiotics.

• Prolonged storage time: encapsulation protects probiotic bacteria from external undesirable factors such as oxygen, temperature, light, etc.
• Easy to transport: encapsulation provides a carrier for bacteria, stabilizing the viability of probiotics
• Good survival of encapsulated bacteria
• Encapsulated bacteria can be freeze-dried

Especially for intestinal probiotics, encapsulation can protect them from adverse gastrointestinal environments, promote their effective attachment to intestinal mucosal tissues, and ensuring the controlled and targeted release of the encapsulated probiotics with desired concentrations in the gastrointestinal tract.

Microfluidic Encapsulation Technology

Microfluidic encapsulation system has shown great potential to encapsulate single cells, bacteria, DNA/RNA, and proteins in a high-throughput and controllable manner. For bacteria encapsulation, it has several important advantages, including:

• Simple, quick and reliable encapsulation of bacteria
• High-through and low-cost encapsulation
• Controllable encapsulation conditions such as desirable encapsulation concentrations and nanoparticles with a certain size.
• High encapsulation efficiency
• Good reproducibility

Application of Microfluidic-based Bacteria Encapsulation

• Bacterial detection

A study reported an imaging software to quantify bacteria based on turbidity. In this study, single bacterial cells, E. coli DH 5α, E. sakazakii, and B. subtilis CMCC (B) 63501, were encapsulated in microbeads using a flow-focusing junction. The imaging software was used to detect a change in microbead turbidity caused by bacterial proliferation. This technology allows fast visible imaging with a high detection sensitivity.

• Antimicrobial susceptibility testing

Microfluidic-based bacteria encapsulation has been used in antimicrobial susceptibility testing (AST). Kaushik et al. developed a platform integrating a flow-focusing droplet generation device with on-chip incubation, imaging, and fluorescence detection to investigate the antimicrobial effect of the antibiotic gentamicin on the growth of E. coli.

Fig. 1 Microfluidic bacteria encapsulation and digital analysis.¹

Services at Creative Biolabs

Creative Biolabs, one of the well-recognized experts, is skilled in microfluidic chip development for bacteria encapsulation. With over ten years' extensive experience and advanced microfluidic platforms, our in-home scientists support one-stop top-quality bacteria encapsulation services, including design and development of microfluidic-based bacteria encapsulation system, production of the nanoparticles with versatile and controllable structures for bacteria encapsulation, and microfluidic encapsulation-based bacterial detection. If you are interested in our custom services, please do not hesitate to contact us to discuss your project.

Reference

1. Kao, Calabrese, et al. " Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification " Biosensors 12.4 (2022): 237.

For Research Use Only. Not For Clinical Use.