Creative Biolabs is a world-leading services provider in the field of microfluidic chip development. Equipped with advanced platforms and powerful technologies, we are dedicated to supporting bacteria encapsulation services by developing a high-quality and high-performance microfluidic chip system.
Encapsulation has become an effective method to protect bacteria against severe environmental factors. In the field of probiotic bacteria, encapsulation emerges as a biotechnological strategy that provides several significant advantages over the use of free probiotics.
Especially for intestinal probiotics, encapsulation can protect them from adverse gastrointestinal environments, promote their effective attachment to intestinal mucosal tissues, and ensuring the controlled and targeted release of the encapsulated probiotics with desired concentrations in the gastrointestinal tract.
Microfluidic encapsulation system has shown great potential to encapsulate single cells, bacteria, DNA/RNA, and proteins in a high-throughput and controllable manner. For bacteria encapsulation, it has several important advantages, including:
A study reported an imaging software to quantify bacteria based on turbidity. In this study, single bacterial cells, E. coli DH 5α, E. sakazakii, and B. subtilis CMCC (B) 63501, were encapsulated in microbeads using a flow-focusing junction. The imaging software was used to detect a change in microbead turbidity caused by bacterial proliferation. This technology allows fast visible imaging with a high detection sensitivity.
Microfluidic-based bacteria encapsulation has been used in antimicrobial susceptibility testing (AST). Kaushik et al. developed a dropFAST platform integrating a flow-focusing droplet generation device with on-chip incubation, imaging, and fluorescence detection to investigate the antimicrobial effect of the antibiotic gentamicin on the growth of E. coli. A recent study reported a microfluidic microwell device integrating a microwell-surface-enhanced Raman scattering (SERS) system. The microwell-SERS system allows bacteria encapsulation in a miniaturized microwell with an increased effective bacteria concentration, thus enabling highly sensitive bacteria detection and rapid AST.
Fig.1 The Microwell-SERS system. (Huang, 2020)
Creative Biolabs, one of the well-recognized experts, is skilled in microfluidic chip development for bacteria encapsulation. With over ten years' extensive experience and advanced microfluidic platforms, our in-home scientists support one-stop top-quality bacteria encapsulation services, including design and development of microfluidic-based bacteria encapsulation system, production of the nanoparticles with versatile and controllable structures for bacteria encapsulation, and microfluidic encapsulation-based bacterial detection. If you are interested in our custom services, please do not hesitate to contact us to discuss your project.
For Research Use Only. Not For Clinical Use.